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. 2024 Jan 29;13(1):59–68. doi: 10.1007/s13668-024-00519-x

Table 2.

Summary of the studies on the association between aflatoxin exposure and child growth parameters

Country Study design Study population Aflatoxin type Analysis technique Detection rate Outcome Finding Reference
Nepal Longitudinal birth cohort 1675 mother and infants (from pregnancy to 2 years of age follow-up)

AFB1-lysine

AFM1

HPLC

1.54 pg/mg

0.04–315.99 ng/L (%94.1)

LAZ, WAZ, WLZ z-scores, length, knee-heel length and stunting AFB1-lysine adduct concentrations were significantly associated with changes in LAZ, length, knee-heel length and WAZ z-scores. Serum aflatoxin concentrations were associated with stunting [49]
Zambia Cross-sectional 400 mothers with children aged 6–24 months AFB1-lys LC–MS/MS NR HAZ, WAZ and WHZ z-scores, stunting, underweight, and wasting AFB1-lys level were found to be significantly associated with child stunting [24]
Tanzania RCT Intervention group n = 150, control group n = 150 Total AFs (AFB1, AFB2, AFG1 and AFG2) HPLC NR WAZ z-score, dietary assessment Mean concentration of AFs was significantly lower in the intervention than control group. Mean WAZ z-score difference between the groups was 0.57 (p < 0.05) [50]
Kenya RCT Intervention group n = 489, control group n = 392 (mother and infants) AFB1-lys HPLC

Intervention group: 2.69 pg/mL

Control group: 2.74 pg/ mL

LAZ z-score, stunting and child serum AFB1-lysine adduct The intervention significantly reduced endline in serum AFB1-lysine adduct levels but had no effect on endline LAZ or stunting [51]
Nepal Longitudinal cohort 85 children (15, 24, and 36 months follow-up) AFB1-lys UPLC 3.62 pg/mg LAZ, WAZ and WLZ z-scores The chronic AF exposure was not significantly associated with anthropometric z-scores [52]
Pakistan Cross-sectional 150 children aged 3–5 years AFM1 ELISA 0.09 ± 0.32 ng/ml HAZ, WAZ, and WHZ z-scores A non-significant correlation was recorded between urinary AFM1 levels and the evaluated anthropometric indices [53]
Tanzania Longitudinal cohort

143 infants

(1st, 3rd and 5th months follow-up)

AFM1

(breast milk)

HPLC AFM1 levels ranging from 0.01 to 0.55 ng/ml WAZ, HAZ and WHZ z-scores A significant association was observed between AFM1 exposure levels and WAZ or HAZ z-scores [2]

AF aflatoxin, HAZ height-for-age, HPLC high-performance liquid chromatography, LAZ length-for-age, LC–MS/MS liquid chromatography-mass spectrometry tandem mass spectrometry, NR not reported, UPLC ultra performance liquid chromatography, RCT randomized controlled trial, WAZ weight-for-age, WHZ weight-for-height, WLZ weight-for-length