FIG. 7.

Transcriptional pulse of the PGK1 and MFA2 mRNAs in pan mutant and wild-type yeast strains. (A) Wild-type yeast strain YAS2286 and pan mutant yeast strain YAS2287 were pregrown in raffinose and sucrose-containing medium and then shifted to galactose (Gal)-containing medium to induce PGK1pG mRNA. Time points were taken at 0, 4, 8, and 12 min following the galactose (Gal) shift. Lanes 1 and 12, DNA markers (M) with sizes (in nucleotides) indicated to the left; lanes 2 and 7, PGK1pG mRNA treated with RNase H and oligo(dT) to remove the poly(A) tail (A₀); lanes 13 and 14, the wild-type (WT) and pan mutant PGK1pG mRNA produced after a 12-min galactose induction were electrophoresed side by side to more easily visualize poly(A) tail length differences. (B) Transcriptional pulse was carried out as described above for panel A, except MFA2pG mRNA was induced in the wild-type yeast strain YAS2284 and the pan mutant yeast strain YAS2285.