TABLE 2.
Maximal mRNA poly(A) tail lengths
| Strain | Relevant genotype | Maximal poly(A) tail lengtha in endogenous mRNA
|
||
|---|---|---|---|---|
| RPL46 | PGK1 | MFA2 | ||
| YAS306 | PAN | 55 ± 5 | 61 ± 3 | 71 ± 2 |
| YAS1254 | PAB1 | 57 ± 6 | 63 ± 1 | 72 ± 6 |
| YAS2283 | pan2Δ pan3 | 76 ± 3 | 75 ± 3 | 74 ± 5 |
| YAS1943 | pan3Δ | 74 ± 2 | 74 ± 4 | 75 ± 5 |
| YAS1942 | pan2Δ | 75 ± 2 | 76 ± 2 | 75 ± 5 |
| YAS1255 | pab1-55 | 74 ± 1 | 74 ± 4 | 77 ± 7 |
a
Maximal poly(A) tail lengths were quantified by using Molecular Dynamics PhosphorImager and ImageQuant software. Values are the averages ± standard deviations from three experiments. Poly(A) tail lenths were calculated as described in Materials and Methods.