Fig. 1.

Establishment of FLNB^{G1586R/G1586R} mice using CRISPR/Cas9. a Diagrammatic representation demonstrating that FLNB c.4756G>A was introduced into the genomic DNA using CRISPR/Cas9. b qPCR analysis of FLNB transcription in the bone marrow cells of FLNB^WT/WT mice, FLNB^WT/G1586R mice and FLNB^{G1586R/G1586R} mice (male, 33-week to 34-week, three mice for each group). c-d Western blot analysis of Filamin B and GAPDH expression in the bone marrow cells of FLNB^WT/WT mice, FLNB^WT/G1586R mice and FLNB^{G1586R/G1586R} mice (male, 33-week to 34-week, three mice for each group). The expression level of Filamin B was significantly down-regulated in the FLNB^WT/G1586R mice and FLNB^{G1586R/G1586R} mice, compared to FLNB^WT/WT mice.