Table 2.

k13 mutations among samples collected during TES in the eight regions of Tanzania from 2016 to 2021

Year	Region	#Sequenced	k13* (%)	k13** (wild-type) (%)	k13** (mutant) (%)	k13 ** (SYN mutation)	k13** (NS mutation)
2016	Kigoma	118	101 (85.6)	95 (94.1)	6 (5.9)	3 (2-R471 & C469)	3 (2-Q613E &I416V)
	Morogoro	104	96 (92.3)	92 (95.8)	4 (4.2)	2 (R471 & R539)	2 (E433D & A578S)
	Pwani	92	79 (85.9)	75 (94.9)	4 (5.1)	4 (P417, C469 & 2-V487	0
	Tanga	109	100 (91.7)	95 (95.0)	5 (5.0)	4 (P417 & 3-C469)	1 (R471S)
2018	Kigoma	111	96 (86.5)	96 (100.0)	0 (0.0)	0	0
	Morogoro	99	87 (87.9)	84 (96.6)	3 (3.4)	3 (G538 & 2-R539)	0
	Pwani	97	79 (81.4)	76 (96.2)	3 (3.8)	3 (S624)	0
	Tanga	119	97 (81.5)	97 (100.0)	0 (0.0)	0	0
2019	Mbeya	95	90 (94.7)	86 (95.6)	4 (4.4)	4 (P417, 2- C469 & R539)	0
	Mtwara	93	88 (94.6)	84 (95.5)	4 (4.5)	3 (C469 & 2-F505)	1 (P475S)
	Tabora	111	96 (86.5)	94 (98.0)	2 (2.0)	2 (P417)	0
	Mwanza	104	79 (76.0)	79 (100.0)	0 (0.0)	0	0
2020	Pwani	97	83 (85.6)	82 (98.8)	1 (1.2)	1 (R539)	0
	Kigoma	103	70 (68.0)	66 (94.3)	4 (5.7)	4 (P417)	0
	Morogoro	96	95 (99.0)	91 (95.8)	4 (4.2)	3 (P417)	1 (R622I)
	Tanga	116	100 (86.2)	100 (100.0)	0 (0.0)	0	0
2021	Mbeya	48	43 (89.6)	43 (100.0)	0 (0.0)	0	0
	Mtwara	87	87 (100.0)	87 (100.0)	0 (0.0)	0	0
	Mwanza	106	96 (90.6)	96 (100.0)	0 (0.0)	0	0
	Tabora	110	107 (97.3)	106 (99.1)	1 (0.9)	1 (Y558)	0
Total		2015	1769 (87.8)	1724 (97.5)	45 (2.5)	37 (2.1%)	8 (0.4%)

k13 = P. falciparum kelch 13, SYN synonymous mutations, and NS non-synonymous mutations

^*number of samples that passed the sequencing quality score criteria

^**number of samples with either wild-type or mutant genotypes and their corresponding proportions where appropriate