Fig. 8.

A natural biofilm-forming variant is completely attenuated in a pneumonic tularaemia mousemodel, but a reverted isolate displays wild-type virulence. Mice were challenged intranasally with doses ranging from 1 to 10⁴ c.f.u for the LVS wild-type (parent), isolate #15 and #15R-1. Displayed are the survival curves for groups of 10 mice following the mice for 21 days post challenge. The accompanying LD₅₀ values and the median time-to-death calculated from these data are displayed in Table 5.