Table 1.
Bacterial strains used in this study
|
Strain |
Strain characteristics |
Source |
|---|---|---|
|
Escherichia coli NEB5-α |
Used for routine plasmid construction in this study |
New England Biolabs |
|
FRAN244 |
Schu S4 (type A1) |
BEI (NR-10492) [20] |
|
244 BF-1 |
Grey variant; biofilm-forming isolate of FRAN244 |
[28] |
|
FRAN255 |
Isolate from human pleura in 2015 (type B2) |
BRMR* [40] |
|
255 BF-1 |
Grey variant; biofilm-forming isolate of FRAN255 |
[28] |
|
255 BF-4 |
Grey variant; biofilm-forming isolate of FRAN255 |
[28] |
|
LVS |
Live vaccine strain |
USAMRIID Repository |
|
LVS isolate #11 |
Grey variant; non-biofilm-forming isolate of LVS |
[28] |
|
LVS isolate #13 |
Grey variant; non-biofilm-forming isolate of LVS |
[28] |
|
LVS isolate #14 |
Grey variant; non-biofilm-forming isolate of LVS |
[28] |
|
LVS isolate #15 |
Grey variant; biofilm-forming isolate of LVS |
[28] |
|
LVS isolate #22 |
Grey variant; biofilm-forming isolate of LVS |
[28] |
|
LVS isolate #26 |
Grey variant; biofilm-forming isolate of LVS |
[28] |
|
LVS isolate #27 |
Grey variant; biofilm-forming isolate of LVS |
[28] |
|
LVS isolate #38 |
Grey variant; biofilm-forming isolate of LVS |
[28] |
|
LVS ΔwbtJ |
In-frame deletion of FTL_0602 in LVS |
This study |
|
LVS ΔwbtJ/pMP814 |
In-frame deletion of FTL_0602 in LVS with pMP814 (KanR) |
This study |
|
LVS ΔwbtJ/pKM43 |
In-frame deletion of FTL_0602 in LVS with pKM43 (KanR) |
This study |
|
LVS isolate #15/pMP814 |
LVS isolate #15 with pMP814 (KanR) |
This study |
|
LVS isolate #15/pKM43 |
LVS isolate #15 with pKM43 (KanR) |
This study |
|
#15-R1 |
Reverted colony from LVS isolate #15; blue variant, non-biofilm-forming |
This study |
|
#15-R2 |
Reverted colony from LVS isolate #15; blue variant, non-biofilm-forming |
This study |
|
#15-R3 |
Reverted colony from LVS isolate #15; blue variant, non-biofilm-forming |
This study |
|
Isolate #15 - MP-1 to 30 |
Strains isolated from mice challenged with LVS isolate #15 |
This study |
|
LVS ΔwbtJ – MP-1 to 5 |
Strains isolated from mice challenged with LVS ΔwbtJ |
This study |
*Biodefense Reference Material Repository.