Table 3.
SNP analysis of biofilm-forming variants to identify mutated genes
|
Strain |
Position |
TYPE |
REF |
ALT |
Strand |
NT pos |
AA pos |
Effect |
Mutation |
Gene |
Product |
|---|---|---|---|---|---|---|---|---|---|---|---|
|
244 BF-1 |
1 503 971 |
snp |
G |
A |
– |
434/726 |
145/241 |
missense |
Ser145Phe |
wbtJ |
dTDP-4-amino-4,6-dideoxyglucose formyltransferase |
|
255 BF-1 |
551 888 |
snp |
A |
T |
+ |
1368/1686 |
456/561 |
missense |
Leu456Phe |
yheS |
putative ABC transporter ATP-binding protein YheS |
|
1 016 152 |
snp |
A |
T |
– |
13/258 |
5/85 |
missense |
Leu5Ile |
hypothetical protein |
||
|
1 099 427 |
snp |
T |
A |
+ |
72/141 |
24/46 |
synonymous |
Pro24Pro |
hypothetical protein |
||
|
1 429 136 |
insert |
T |
TA |
+ |
433/447 |
145/148 |
frameshift |
Tyr145fs |
IS630 family transposase ISFtu1 |
||
|
1 503 160 |
delete |
TC |
T |
– |
370/726 |
124/241 |
frameshift |
Glu124fs |
wbtJ |
dTDP-4-amino-4,6-dideoxyglucose formyltransferase |
|
|
1 608 120 |
delete |
AT |
A |
intergenic |
|||||||
|
255 BF-4 |
460 860 |
snp |
C |
G |
+ |
383/1347 |
128/448 |
nonsense |
Ser128* |
dtpA1 |
Dipeptide and tripeptide permease A |
|
1 249 956 |
snp |
T |
G |
– |
441/465 |
147/154 |
synonymous |
Ile147Ile |
dtpD |
Dipeptide permease D |
|
|
1 503 065 |
insert |
C |
CT |
– |
465/726 |
155/241 |
frameshift |
Glu156fs |
wbtJ |
dTDP-4-amino-4,6-dideoxyglucose formyltransferase |
|
|
1 643 954 |
snp |
T |
C |
unknown |
intergenic |
||||||
|
1 736 592 |
snp |
A |
C |
unknown |
intergenic |