Table 5.
LD50 and TTD analysis for mice challenged intranasally with LVS variants and a ΔwbtJ mutant
|
LVS wild-type |
Isolate #15 |
#15-R1 |
Δ wbtJ |
Δ wbtJ + pKM43 |
||
|
LD50 (c.f.u.) |
509 |
>45 000 |
815 |
>49 000 |
933 |
|
|
Median TTD (Days) |
1–10 |
>21 |
>21 |
>21 |
>21 |
>21 |
|
10–100 |
>21 |
>21 |
>21 |
>21 |
>21 |
|
|
100–1000 |
>21 |
>21 |
>21 |
>21 |
>21 |
|
|
1,000–10 000 |
7.5 |
>21 |
7 |
>21 |
9 |
|
|
>10 000 |
6.5 |
>21 |
5 |
>21 |
7.5 |
|
|
Pair-Wise Comparisons (Wald-test) |
vs. LVS wild-type |
P<0.01 |
ns |
P<0.01 |
ns |
P values for comparison among strains were determined by Wald Chi-square tests based on a Cox regression model.
NS, Not Significant (P>0.05); TTD, Time to Death.