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. 2024 Mar 9;15:2161. doi: 10.1038/s41467-024-46449-8

Fig. 2. Subcutaneous B12 supplementation efficiently restores B12 serum levels in anemic mice and results in enhanced M. tuberculosis virulence.

Fig. 2

a Experimental design and timelines of the different B12 treatments, B12 subcutaneous supplementation, and M. tuberculosis infection, in each SCID mice group. b B12 serum levels in animal groups described in panel (a) after sacrifice at the humane endpoint. B12 levels remained significantly decreased in animals treated with B12 restricted diets relative to the control group throughout the curse of the experiment. Note that weekly B12 supplementation restored standard B12 serum levels in mice. Graph data are mean ± SD of n = 6 biological replicates. Statistical analysis was performed using One-Way ANOVA and Tukey post-test. c Survival rates from groups of 6 SCID mice for each type of treatment after infection by the intranasal route with M. tuberculosis. Statistical analysis was performed using Log-rank (Mantel-Cox) test. p-values are as follows: ****0.0001 > p; **0.01 > p > 0.001; *0.05 > p > 0.01; ns not significant, p ≥ 0.05.