Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Mar 9;15:2161. doi: 10.1038/s41467-024-46449-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a Expected phenotypes of the M. tuberculosis H37Rv ΔmetH Pr_Ag85ametE::Kan complemented strain compared to the parental ΔmetH strain in presence and absence of B12. b Validation by qRT-PCR of the metE complementation and its stable expression in the complemented mutant M. tuberculosis H37Rv ΔmetH Pr_Ag85ametE::Kan compared to the ΔmetH mutant and the wild type strains. Relative quantity refers to the differential metE gene expression in each strain in comparison with its expression in the wild type H37Rv. Each gene was normalized against sigA expression in each sample. Graphs represents mean ± SD from one experiment with three replicates. Statistical analysis was performed using two-way ANOVA followed by Tukey’s multiple comparisons post-test. p-values are as follows: ****0.0001 > p; ***0.001 > p > 0.0001; *0.05 > p > 0.01. c Experimental growth in liquid (boxed images) and in solid media of the M. tuberculosis H37Rv ΔmetH and the ΔmetH Pr_Ag85ametE::Kan strains in presence and absence of B12. d and f Survival rates from groups of SCID mice fed with normal diet (d) or B12-deficient diet (f), and inoculated by intranasal route with M. tuberculosis H37Rv, its ΔmetH mutant or the ΔmetH Pr_Ag85ametE::Kan complemented strain. Each curve represents the pool from 2 independent experiments (n = 12). Statistical analysis was performed using Log-rank (Mantel-Cox) test. e and g Bacterial loads in the lungs of normal (e) and B12 anemic (g) C57BL/6 mice after 24 h, or 4 weeks infected with M. tuberculosis H37Rv, the ΔmetH knockout, or the ΔmetH Pr_Ag85ametE::Kan Data are mean ± SD of n ≥ 6 biological replicates per mice group. Statistical analysis was performed using unpaired t-test. p-values are as follows: ***0.001 > p > 0.0001; *0.05 > p > 0.01; ns: not significant, p ≥ 0.05. Results show that complementation with a B12-independent metE restores virulence in the M. tuberculosis ΔmetH mutant independently of the B12 status of the host.