Fig. 1. Identification of Mi-2β regulating melanoma cell resistance to immunotherapy.

a, b Mice bearing Mi-2β knockdown or shScramble B16F10 cells were treated with i.p. injection of control IgG (10 mg/kg) or anti-PD-1 (10 mg/kg) antibodies at day 6, 9, 12, 15 and 18 after tumor cell inoculation, tumor volume (n = 5) and tumor weight (n = 5) were measured. c Mouse survival (n = 5) over time. Log-rank test was used to determine P value. d The representative cell populations of CD4⁺ and CD8⁺ are shown. e Tumor-infiltrating lymphocytes in the graft tumor were measured by flow cytometry. e The number of CD4⁺ T cells and CD8⁺ were gated within CD45⁺ T cells (n = 5). f Granzyme B expression in CD8⁺ T was measured and quantified by flow cytometry (n = 5). a, b, e, f Values represent mean ± SD. The unpaired, two tailed t-test. Source data are provided as a Source Data file.