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. 2024 Mar 9;15:2163. doi: 10.1038/s41467-024-46422-5

Fig. 3. Depletion of Mi-2β promotes the transcription of interferon-stimulated genes by promoting chromatin remodeling.

Fig. 3

a The amount of secreted Cxcl9 or Cxcl10 were measured in B16F10 cells with Mi-2β silencing by ELISA (n = 3). b The graft melanomas were isolated and cultured in PBS with the same amount of cells for 4 h (for each group graft melanomas = 5), and then the secreted amount of the chemokines Cxcl9 and Cxcl10 in the culture medium was measured by ELISA. c Genomic distribution of DARs and binding consensus of the TFs. d ATAC-seq bedgraph panels of the gene locus showing the peak locations relative to the TSS. The panels were compared with ATAC signals between scramble and Mi-2β knockdown B16F10 cells. e ChIP assays were performed to detect Mi-2β binding on the promoter of Cxcl9 and Cxcl10 genes in both shScramble and Mi-2β knockdown B16F10 cells, with IP by anti-Stat1 was used as the positive binding control (n = 3). a, b, e Values represent mean ± SD. The unpaired, two tailed t-test. Source data are provided as a Source Data file.