Table 2.

Primer sequences used for real-time PCR.

Gene	Product length	Primer sequences (Oligo sequence 5' → 3')	Melt temperature (°C)
P53	125	Forward: ACCTATGGAAACTACTTCCTGA Reverse: TTCATCTGGACCTGGGTCTTCA	73.66
CTR1	293	Forward: TGCCATTGACATCAAACTCTATGGC Reverse: GGCTCTAAGATGATGGAGAAGGA	70.5
Bcl2	110	Forward: GGAGGCTGGGATGCCTTTGTGGA Reverse: CAAGCTCCCACCAGGGCCAAACT	79.15
P21	188	Forward: AGAGGAGGCGCCATGTCAGAAC Reverse: AGTGGTGTCTCGGTGACAAAGT	74.56
Gapdh	117	Forward: AGGGCTGCTTTTAACTCTGGT Reverse: GCCATGGAATTTGCCATGGGT	85.87