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. 2024 Mar 9;7:294. doi: 10.1038/s42003-024-06006-x

Fig. 3. Mtb-infection of humanized NSG-SGM3 mice.

Fig. 3

A Reconstitution of human immune cells in splenocytes (upper panels) and PBMC (lower panels) from humanized NSG-SGM3 mice. NSG-SGM3 mice were irradiated and intravenously injected with 2 × 105 hCD34+ HSC. After 10–12 weeks, reconstitution of human T cells, B cells, myeloid cells, and natural killer (NK) cells within human CD45-gated population was analyzed by flow cytometry. B Statistics of different immune cells before and after infection. C Five mice were sacrificed to check Mtb infection in lungs and spleens by determining the organ CFUs by plating serially diluted organ homogenates on 7H10 agar plates as described above.