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. 2024 Mar 9;15:2165. doi: 10.1038/s41467-024-46509-z

Fig. 6. LSD1 promotes R-loop formation at ALT telomeres.

Fig. 6

a Representative images and b quantification of R-loop (indicated by 2× HBD-GFP) on telomeres after dimerization of LSD1ΔIDR, LSD1ΔIDR, 3KE to Halo-TRF1 in U2OS cells (n = three independent experiments, one-way ANOVA). Because the telomeres are not labeled, LSD1 foci after adding TFH for 0.5 h (shortly after dimerization completion) were used to infer telomere locations. c DRIP assay shows R-loop level on telomeres from chromatin 10q, 13q, 15q after siLSD1 or siTERRA (100 nM, 48 h) in FokI stable cell line (n = three experiments, one-way ANOVA). d DRIP assay shows R-loop level on telomeres from chromatin 10q, 13q, 15q after dimerization of LSD1ΔIDR and LSD1ΔIDR, 3KE to Halo-TRF1 in siCtr or siTERRA U2OS cells (n = three independent experiments, two-way ANOVA). e Fluorescent images of condensates after combination of ATTO594-labeled Rad51AP1 (30 μM), FITC-labeled LSD1ΔIDR (50 μM) and 8× TERRA (50 μM). f ChIP-qRCR assay detecting Rad51AP1 binding to telomere DNA from indicated chromatin in siCtr-, siLSD1-, and siTERRA- transfected U2OS cells (n= three independent experiments, one-way ANOVA). g DRIP assay shows R-loop level on telomeres from chromatin 10q, 13q, 15q after dimerization of LSD1ΔIDR to Halo-TRF1 in U2OS RAD51AP1 WT and KO cells (n = three independent experiments, two-way ANOVA). Error bars are mean ± SEM. NS not significant. Source data are provided as a Source Data file.