Extended Data Fig. 4: Knockout of TRAIL in 1928ζ CAR-T cells significantly impairs antitumor cytolytic activity.

(a) Schematic for the CRISPR/Cas9-mediated knockout (KO) of TRAIL in human CD8⁺ T cells expressing a 1928ζ CAR. (b) Cytolytic activity of TRAIL-KO versus wild type (WT)-TRAIL 1928ζ CAR-T cells or T cells transduced with tEGFR alone against Nalm6/mCherry at indicated effector to target (E:T) ratios. Data shown as mean ± s.e.m. using n=3 biological replicates per condition. Statistical comparisons performed using a one-way ANOVA. ns = not significant, P>0.05. (c) Table displaying the measured frameshift insertion-deletion (Indel) frequency in TRAIL for each T cell group used in the cytolytic assay. Data shown as mean ± s.e.m. from n=3 technical replicates.