Figure 1: Repeated administration of CpG-DNA induced characteristics of hyperinflammatory disease and inflammasome signalling.

(A) Mice were treated over a 10-day period with CpG-DNA (ODN 1826, 2mg/kg), or vehicle (PBS), by intraperitoneal injection on days 0, 2, 4, 7 and 9. (B) Animal weight was measured daily over the course of the study. Animal weights presented as a percentage of weight on day 0. Animals were sacrificed on day 10 and hyperinflammatory readouts were recorded. (C) Splenic weight normalised to body weight from CpG-DNA (CpG) or PBS injected mice (n=5). (D) Representative images from (C). (E) Plasma levels of ferritin (n=5). (F-J) Plasma concentration of IFNγ (F), IL-6 (G), IL-10 (H) and TNF (I), IL-18 (J) (n=5). (K) Western blot of homogenised spleens from PBS or CpG treated animals were blotted for inflammasome components NLRP3, caspase-1, GSDMD and IL-1β (n=5). Data represent the mean ± SEM. *, P<0.05, **, P<0.01, ***, P<0.001, ****, P<0.0001 determined by an unpaired Student’s t-test.