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[Preprint]. 2024 Mar 1:2024.02.27.582284. [Version 1] doi: 10.1101/2024.02.27.582284

Figure 2: The NLRP3 inflammasome is dispensable for the development of CpG-induced hyperinflammation.

Figure 2:

(A) Mice received 5 doses of CpG-DNA (CpG, ODN 1826, 2 mg/kg), along with vehicle (PBS) or MCC950 (MCC, 50 mg/kg). (B) Splenic weight normalised to body weight from CpG-DNA (CpG) or PBS injected mice (n=5). (C) Representative images from (B). (D) Plasma levels of ferritin (n=5). (E) H&E staining of spleen in mice treated with PBS/PBS, CpG/PBS or CpG/MCC. RP = red pulp, WP = white pulp. White arrows denote changes to normal splenic architecture and perturbations to red pulp. Scale bar represents 200 μm. (F-J) Plasma concentration of IFNγ (F), IL-6 (G), IL-10 (H) and TNF (I), IL-18 (J) (n=5). Data represent the mean ± SEM. *, P<0.05, **, P<0.01, ***, P<0.001, ****, P<0.0001 determined by one-way ANOVA with Tukey’s multiple comparisons test.