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[Preprint]. 2024 Mar 1:2024.02.27.582284. [Version 1] doi: 10.1101/2024.02.27.582284

Figure 3. Canonical inflammasome activation is dispensable for the development of CpG-induced MAS.

Figure 3.

(A) Mice received 5 doses of CpG (CpG, ODN 1826, 2 mg/kg), along with daily injections of vehicle (Veh; PBS, 5% DMSO) or VX765 (VX, 100 mg/kg) over 10 days. (B) Splenic weight normalised to body weight in mice treated with PBS/PBS, CpG/Veh or CpG/VX (n=5). (C) Representative images from (B). (D) Plasma levels of ferritin in mice treated with PBS/PBS, CpG/Veh or CpG/VX (n=5). (E) H&E staining of spleen in mice treated with PBS/PBS, CpG/Veh or CpG/VX. RP= red pulp, WP= white pulp. White arrows denote changes to normal splenic architecture and perturbations to red pulp. (F-J) Plasma concentrations of IFNγ (F), IL-6 (G), IL-10 (H), TNF (I), and IL-18 (J) in mice treated repeatedly with PBS/PBS, CpG/Veh or CpG/VX (n=5). Data represent mean ± SEM. *, P<0.05, **, P<0.01, ***, P<0.001 determined by a one-way ANOVA with Tukey’s multiple comparisons test.