Figure 5. Directing protein localization with split-mNG2.

A. Schematic illustrating use of the split-mNG2 system to sequester proteins of interest on mitochondria. B–G. Representative images of krt8-mNG2₁₁ embryos injected with mNG2_1–10 (B–D) or mito-mNG2_1–10 (E–G) mRNA. Images were acquired from the tail fin epidermis at 48 hours post-fertilization (hpf). Mitochondria were labeled with MitoTracker dye. Scale bars, 50 μm. H–J. Representative images of the tails of uninjected control embryos (H) krt8-mNG2₁₁ embryos injected with mito-mNG2_1–10 mRNA (I, J). Images were acquired at 48 hpf. Arrows denote blisters. Scale bars, 200 μm.