Figure 1. Gene knock-out of cd44a results in significant reduction in airineme extension.

(A, B) Merged time-lapse frames over 500 mins display airinemes. Red arrowheads mark airineme vesicles. (C, D) The extension of airinemes was significantly decreased in embryos injected with cd44a sgRNA/Cas9, (F₂, ₁₂) = 172.8, P<0.0001, 5 larvae each). Statistically significant results were evaluated using a One-way ANOVA, followed by a Tukey’s HSD post hoc test. Scale bars represent 20μm. Error bars denote mean ± SEM. Images were overexposed to visualize thin airineme filaments.