Table 1.
Reagents used for cell stimulation throughout this study.
| Reagent | Manufacturer | Catalog Number | Stock Solution | Storage Temperature | Concentration Added to Cells (diluted in cell media) |
|---|---|---|---|---|---|
| PMA | Millipore Sigma | P8139 | 437 μM in DMSO | −20° C | 100 nM |
| FIPI | Millipore Sigma | 528245 | 750 μM in DMSO | −20° C | 750 nM |
| DiC8 | EMD Millipore | 317505 | 30 mM in methanol, dried. Resuspended in 50 mL methanol before use. | −80° C | 150 μM |
| OAG | EMD Millipore | 495414 | 30 mM in methanol, dried. Resuspended in 20 mL DMSO before use. | −80° C | 150 μM |
| PDBu | Sigma | P1269 | 10 mM in DMSO | −80° C | 5 μM |
| Rapamycin | Sigma | 553210 | 1 mM in DMSO | −20° C | 1 μM |
| Carbachol | Fisher | AC10824 | 50 mM in dH2O | −20° C | 5 μM, 10 μM, or 100 μM |
| Atropine | Sigma | A0257 | 25 mM in dH2O | 4° C | 5 μM |
| ATP | Sigma | 10127523001 | 100 mM in 100 mM MgCl2 + 200 mM Tris base | −20° C | 100 μM |
| Histamine | Fisher | AC15062 | 100 mM in dH2O | −20° C | 100 μM |