Table 2.
Plasmids used in this study. All genes are Homo sapiens unless indicated otherwise. The relevant amino acid residues are noted and colored accordingly. Mutations are described by the position of the residues in the full-length protein and are highlighted in the sequence. Gray amino acid sequences indicate linkers.
| Shorthand Name | Full Name | Reference | Sequence |
|---|---|---|---|
| NES-PABD-Spo20 | X.leavis map2k1.L32-44-EGFP-S. cerevisiae Spo20p51-91 | (Zeniou-Meyer et al., 2007; Zhang et al., 2014) | MALQKKLEELELDEAPVATMVSKGEELFTGVVPILVELDGDVNGHK FSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFS RYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEG DTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSK DPNEKRDHMVLLEFVTAAGITLGMDELYKGLRSRASIMDNCSGSR RRDRLHVKLKSLRNKIHKQLHPNCRFDDATKTS* |
| PASS | EGFP-M. musculus PKIa34-51-S. cerevisiae Spo20p51-91 | (Zhang et al., 2014; Zeniou-Meyer et al., 2007) |
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMP EGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNI LGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHY QQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAA GITLGMDELYKSGLRSRANSNELALKLAGLDINKTESRMDNCSASR RRDRLHVKLKSLRNKIHKQLHPNCRFDDATKTS* |
| NES-flex-PABD-Spo20 | X.leavis map2k1.L32-44-EGFP-SGGGSGGS-S. cerevisiae Spo20p51-91 | This Study | MALQKKLEELELDEAPVATMVSKGEELFTGVVPILVELDGDVNGHK FSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFS RYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEG DTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSK DPNEKRDHMVLLEFVTAAGITLGMDELYKSGGGSGGSMDNCSGS RRRDRLHVKLKSLRNKIHKQLHPNCRFDDATKTS* |
| NES-PABDx2-Spo20 | X.leavis map2k1.L32-44-EGFP-S. cerevisiae Spo20p51-91- S. cerevisiae Spo20p51-91 | (Bohdanowicz et al., 2013) | MALQKKLEELELDEAPVATMVSKGEELFTGVVPILVELDGDVNGHK FSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFS RYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEG DTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSK DPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAMDNCSGSRR RDRLHVKLKSLRNKIHKQLHPNCRFDDATKTSMDNCSGSRRRDRL HVKLKSLRNKIHKQLHPNCRFDDATKTS* |
| NESx2-PABDx2-Spo20 | X.leavis map2k1.L32-44-X.leavis map2k1.L32-44-EGFP-S. cerevisiae Spo20p51-91- S. cerevisiae Spo20p51-91 | This Study | MALQKKLEELELDEAGGSGMALQKKLEELELDEAPVATMVSKGEE LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKL PVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNY NSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDG PVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELY KSGLRSRAMDNCSGSRRRDRLHVKLKSLRNKIHKQLHPNCRFDDA TKTSMDNCSGSRRRDRLHVKLKSLRNKIHKQLHPNCRFDDATKTS* |
| Nir1-LNS2 | NeonGreen-Nir1613-897 | This Study |
MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGY EELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMV DGSGYQVHRTMQFEDGASLTVNYRYTYEGSHIKGEAQVKGTGFP ADGPVMTNSLTAADWCRSKKTYPNDKTIISTFKWSYTTGNGKRYR STARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK AFTDVMGMDELYKGGSGGMPANPREKWLRKRTQVKLRNVTANH RANDVIAAEDGPQVLVGRFMYGPLDMVALTGEKVDILVMAEPSSG RWVHLDTEITNSSGRITYNVPRPRRLGVGVYPVKMVVRGDQTCAM SYLTVLPRGMECVVFSIDGSFAASVSIMGSDPKVRPGAVDVVRHW QDLGYMILYITGRPDMQKQRVVSWLSQHNFPQGMIFFSDGLVHDP LRQKAIFLRNLMQECFIKISAAYGSTKDISVYSVLGLPASQIFIVGRPT KKYQTQCQFLSEGYAAHLAALEASHRSRPKKN* |
| Nir2-LNS2 | NeonGreen-Nir2896-1216 | This Study |
MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGY EELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMV DGSGYQVHRTMQFEDGASLTVNYRYTYEGSHIKGEAQVKGTGFP ADGPVMTNSLTAADWCRSKKTYPNDKTIISTFKWSYTTGNGKRYR STARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK AFTDVMGMDELYKGGSGGMSPAFPREKWQRKRTQVKIRNVTSNH RASDTVVCEGRPQVLSGRFMYGPLDVVTLTGEKVDVYIMTQPLSG KWIHFGTEVTNSSGRLTFPVPPERALGIGVYPVRMVVRGDHTYAE CCLTVVARGTEAVVFSIDGSFTASVSIMGSDPKVRAGAVDVVRHW QDSGYLIVYVTGRPDMQKHRVVAWLSQHNFPHGVVSFCDGLTHD PLRQKAMFLQSLVQEVELNIVAGYGSPKDVAVYAALGLSPSQTYIV GRAVRKLQAQCQFLSDGYVAHLGQLEAGSHSHASSGPPRAALGK SSYGVAAPVDFLRKQSQLLRSRGPSQA* |
| Nir3-LNS2 | mEGFP-Nir3925-1209 | This Study |
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMP EGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNI LGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHY QQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAA GITLGMDELYKGGGGSHMPSKPREKWQRKRTHVKLRNVTANHRI NDALANEDGPQVLTGRFMYGPLDMVTLTGEKVDVHIMTQPPSGE WLYLDTLVTNNSGRVSYTIPESHRLGVGVYPIKMVVRGDHTFADSY ITVLPKGTEFVVFSIDGSFAASVSIMGSDPKVRAGAVDVVRHWQDL GYLIIYVTGRPDMQKQRVVAWLAQHNFPHGVVSFCDGLVHDPLRH KANFLKLLISELHLRVHAAYGSTKDVAVYSAISLSPMQIYIVGRPTKK LQQQCQFITDGYAAHLAQLKYSHRARPARN* |
| Nir1-613-630 | Nir1613-630-NeonGreen | This Study | MPANPREKWLRKRTQVKLRGGSGGMVSKGEEDNMASLPATHEL HIFGSINGVDFDMVGQGTGNPNDGYEELNLKSTKGDLQFSPWILV PHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGASL TVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSK KTYPNDKTIISTFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKN QPMYVFRKTELKHSKTELNFKEWQKAFTDVMGMDELYK* |
| Nir1-631-894 | Nir1631-894-NeonGreen | This Study |
MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGY EELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMV DGSGYQVHRTMQFEDGASLTVNYRYTYEGSHIKGEAQVKGTGFP ADGPVMTNSLTAADWCRSKKTYPNDKTIISTFKWSYTTGNGKRYR STARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK AFTDVMGMDELYKGGSGGNVTANHRANDVIAAEDGPQVLVGRFM YGPLDMVALTGEKVDILVMAEPSSGRWVHLDTEITNSSGRITYNVP RPRRLGVGVYPVKMVVRGDQTCAMSYLTVLPRGMECVVFSIDGS FAASVSIMGSDPKVRPGAVDVVRHWQDLGYMILYITGRPDMQKQR VVSWLSQHNFPQGMIFFSDGLVHDPLRQKAIFLRNLMQECFIKISA AYGSTKDISVYSVLGLPASQIFIVGRPTKKYQTQCQFLSEGYAAHLA ALEASHRSRP* |
| C1ab-Prkd1 | X.leavis map2k1.L32-44-mCherry-M. musculus Prkd1138-343 | (Kim et al., 2011) | MALQKKLEELELDEAPVATMVSKGEEDNMAIIKEFMRFKVHMEGS VNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFM YGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDS SLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDIT SHNEDYTIVEQYERAEGRHSTGGMDELYKSGLRSRAQASNSTSED FQIRPHALFVHSYRAPAFCDHCGEMLWGLVRQGLKCEGCGLNYH KRCAFKIPNNCSGVRRRRLSNVSLTGLGTVRTASAEFSTSVPDEPL LSPVSPGFEQKSPSESFIGREKRSNSQSYIGRPIQLDKLLMSKVKV PHTFVIHSYTRPTVCQFCKKLLKGLFRQGLQCKDCRFNCHKRCAP KVPNNCLGEVTINGELLSPGAES* |
| FKBP-PJ-Dead | mCherry-FKBP1A3-109-S. cerevisiae Sac12-517, C392S - INPP5E214-644, D556A,C641A | (Hammond et al., 2012) |
MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLS FPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPS DGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDA EVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHS TGGMDELYKSGLRSRSAAAGAGGAARAALGVQVETISPGDGRTFP KRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWE EGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLE SAGGSAGGSAGGSAGGSAGGPRAQASRSLDATGPIVYVQNADGI FFKLAEGKGTNDAVIHLANQDQGVRVLGAEEFPVQGEVVKIASLM GFIKLKLNRYAIIANTVEETGRFNGHVFYRVLQHSIVSTKFNSRIDSE EAEYIKLLELHLKNSTFYFSYTYDLTNSLQRNEKVGPAASWKTADE RFFWNHYLTEDLRNFAHQDPRIDSFIQPVIYGYAKTVDAVLNATPIV LGLITRRSIFRAGTRYFRRGVDKDGNVGNFNETEQILLAENPESEKI HVFSFLQTRGSVPIYWAEINNLKYKPNLVLGENSLDATKKHFDQQK ELYGDNYLVNLVNQKGHELPVKEGYESVVHALNDPKIHYVYFDFH HECRKMQWHRVKLLIDHLEKLGLSNEDFFHKVIDSNGNTVEIVNEQ HSVVRTN[S]MDCLDRTNVVQSVLAQWVLQKEFESADVVATGSTW EDNAPLLTSYQNLWADNADAVSVAYSGTGALKTDFTRTGKRTRLG AFNDFLNSASRYYQNNWTDGPRQDSYDLFLGGFRPHTASIKSPFP GGTARGAAAGAGGAGRSDLADYKLRAQPLLVRAHSSLGPGRPRS PLACDDCSLRSAKSSFSLLAPIRSKDVRSRSYLEGSLLASGALLGA DELARYFPDRNVALFVATWNMQGQKELPPSLDEFLLPAEADYAQD LYVIGVQEGCSDRREWETRLQETLGPHYVLLSSAAHGVLYMSLFIR RDLIWFCSEVECSTVTTRIVSQIKTKGALGISFTFFGTSFLFITSHFTS GDGKVAERLLDYTRTVQALVLPRNVPDTNPYRSSAADVTTRFDEV FWFGDFNFRLSGGRTVVDALLCQGLVVDVPALLQHDQLIREMRKG SIFKGFQEPDIHFLPSYKFDIGKDTYDSTSKQRTPSYT[A]RVLYRSR HKGDICPVSYSSCPGIKTSDHRPVYGLFRVKVRPGRDNIPLAAGKF DRELYLLGIKRRISKEIQRQQALQSQNSSTI[A]SVS* |
| FKBP-PJ | mCherry-FKBP1A3-109-S. cerevisiae Sac12-517- INPP5E214-644, C641A | (Hammond et al., 2012) |
MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLS FPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPS DGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDA EVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHS TGGMDELYKSGLRSRSAAAGAGGAARAALGVQVETISPGDGRTFP KRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWE EGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLE SAGGSAGGSAGGSAGGSAGGPRAQASRSLDATGPIVYVQNADGI FFKLAEGKGTNDAVIHLANQDQGVRVLGAEEFPVQGEVVKIASLM GFIKLKLNRYAIIANTVEETGRFNGHVFYRVLQHSIVSTKFNSRIDSE EAEYIKLLELHLKNSTFYFSYTYDLTNSLQRNEKVGPAASWKTADE RFFWNHYLTEDLRNFAHQDPRIDSFIQPVIYGYAKTVDAVLNATPIV LGLITRRSIFRAGTRYFRRGVDKDGNVGNFNETEQILLAENPESEKI HVFSFLQTRGSVPIYWAEINNLKYKPNLVLGENSLDATKKHFDQQK ELYGDNYLVNLVNQKGHELPVKEGYESVVHALNDPKIHYVYFDFH HECRKMQWHRVKLLIDHLEKLGLSNEDFFHKVIDSNGNTVEIVNEQ HSVVRTNCMDCLDRTNVVQSVLAQWVLQKEFESADVVATGSTWE DNAPLLTSYQNLWADNADAVSVAYSGTGALKTDFTRTGKRTRLGA FNDFLNSASRYYQNNWTDGPRQDSYDLFLGGFRPHTASIKSPFPG GTARGAAAGAGGAGRSDLADYKLRAQPLLVRAHSSLGPGRPRSP LACDDCSLRSAKSSFSLLAPIRSKDVRSRSYLEGSLLASGALLGAD ELARYFPDRNVALFVATWNMQGQKELPPSLDEFLLPAEADYAQDL YVIGVQEGCSDRREWETRLQETLGPHYVLLSSAAHGVLYMSLFIRR DLIWFCSEVECSTVTTRIVSQIKTKGALGISFTFFGTSFLFITSHFTSG DGKVAERLLDYTRTVQALVLPRNVPDTNPYRSSAADVTTRFDEVF WFGDFNFRLSGGRTVVDALLCQGLVVDVPALLQHDQLIREMRKGS IFKGFQEPDIHFLPSYKFDIGKDTYDSTSKQRTPSYTDRVLYRSRHK GDICPVSYSSCPGIKTSDHRPVYGLFRVKVRPGRDNIPLAAGKFDR ELYLLGIKRRISKEIQRQQALQSQNSSTI[A]SVS* |
| FKBP-INPP5E | mCherry-FKBP1A3-109-INPP5E214-644, C641A | (Hammond et al., 2012) |
MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLS FPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPS DGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDA EVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHS TGGMDELYKSGLRSRSAAAGAGGAARAAMGVQVETISPGDGRTF PKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGW EEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLE ARGAAAGAGGAGRSDLADYKLRAQPLLVRAHSSLGPGRPRSPL ACDDCSLRSAKSSFSLLAPIRSKDVRSRSYLEGSLLASGALLGADE LARYFPDRNVALFVATWNMQGQKELPPSLDEFLLPAEADYAQDLY VIGVQEGCSDRREWETRLQETLGPHYVLLSSAAHGVLYMSLFIRR DLIWFCSEVECSTVTTRIVSQIKTKGALGISFTFFGTSFLFITSHFTSG DGKVAERLLDYTRTVQALVLPRNVPDTNPYRSSAADVTTRFDEVF WFGDFNFRLSGGRTVVDALLCQGLVVDVPALLQHDQLIREMRKGS IFKGFQEPDIHFLPSYKFDIGKDTYDSTSKQRTPSYTDRVLYRSRHK GDICPVSYSSCPGIKTSDHRPVYGLFRVKVRPGRDNIPLAAGKFDR ELYLLGIKRRISKEIQRQQALQSQNSSTI[A]SVS* |
| FKBP-Sac1 | mCherry-FKBP1A3-109-S. cerevisiae Sac12-517-INPP5E214-644, D556A C641A | (Hammond et al., 2012) |
MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLS FPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPS DGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDA EVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHS TGGMDELYKSGLRSRSAAAGAGGAARAALGVQVETISPGDGRTFP KRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWE EGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLE SAGGSAGGSAGGSAGGSAGGPRAQASRSLDATGPIVYVQNADGI FFKLAEGKGTNDAVIHLANQDQGVRVLGAEEFPVQGEVVKIASLM GFIKLKLNRYAIIANTVEETGRFNGHVFYRVLQHSIVSTKFNSRIDSE EAEYIKLLELHLKNSTFYFSYTYDLTNSLQRNEKVGPAASWKTADE RFFWNHYLTEDLRNFAHQDPRIDSFIQPVIYGYAKTVDAVLNATPIV LGLITRRSIFRAGTRYFRRGVDKDGNVGNFNETEQILLAENPESEKI HVFSFLQTRGSVPIYWAEINNLKYKPNLVLGENSLDATKKHFDQQK ELYGDNYLVNLVNQKGHELPVKEGYESVVHALNDPKIHYVYFDFH HECRKMQWHRVKLLIDHLEKLGLSNEDFFHKVIDSNGNTVEIVNEQ HSVVRTNCMDCLDRTNVVQSVLAQWVLQKEFESADVVATGSTWE DNAPLLTSYQNLWADNADAVSVAYSGTGALKTDFTRTGKRTRLGA FNDFLNSASRYYQNNWTDGPRQDSYDLFLGGFRPHTASIKSPFPG GTARGAAAGAGGAGRSDLADYKLRAQPLLVRAHSSLGPGRPRSP LACDDCSLRSAKSSFSLLAPIRSKDVRSRSYLEGSLLASGALLGAD ELARYFPDRNVALFVATWNMQGQKELPPSLDEFLLPAEADYAQDL YVIGVQEGCSDRREWETRLQETLGPHYVLLSSAAHGVLYMSLFIRR DLIWFCSEVECSTVTTRIVSQIKTKGALGISFTFFGTSFLFITSHFTSG DGKVAERLLDYTRTVQALVLPRNVPDTNPYRSSAADVTTRFDEVF WFGDFNFRLSGGRTVVDALLCQGLVVDVPALLQHDQLIREMRKGS IFKGFQEPDIHFLPSYKFDIGKDTYDSTSKQRTPSYT[A]RVLYRSRH KGDICPVSYSSCPGIKTSDHRPVYGLFRVKVRPGRDNIPLAAGKFD RELYLLGIKRRISKEIQRQQALQSQNSSTI[A]SVS* |
| PM-FRB | Lyn1-11-MTOR2021-2113-iRFP |
(Hammond et al., 2014) |
MGCIKSKGKDSRSANSGAGAGAGAILSRILWHEMWHEGLEEASRL YFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEA QEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKTSYPYDVPDYAP VATMAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAG SDNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPIT VGFTMRKDAGFIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNS AIRRLQAAETLESACAAAAQEVRKITGFDRVMIYRFASDFSGEVIAE DRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDINYRPVPVTP DLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERL WGLIVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE* |
| Mito-FRB | iRFP-MTOR2021-2113-Fis122-152 |
(Doyle et al., 2023) |
MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGSDN LPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGF TMRKDAGFIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRR LQAAETLESACAAAAQEVRKITGFDRVMIYRFASDFSGEVIAEDRC AEVESKLGLHYPASTVPAQARRLYTINPVRIIPDINYRPVPVTPDLNP VTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGLIV CHHRTPYYVDLDGRQACELVAQVLAWQIGVMEESGLRSRAGGAG AILSRILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMER GPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDL YYHVFRRISKGGSAGGSAQASNSAVDGTAGLVGMAIVGGMALGV AGLAGLIGLAVSKSKS* |
| FKBP-PI4K | mCherry-FKBP1A3-108-PI4KA1102-2103 | (Zewe et al., 2020) |
MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLS FPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPS DGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDA EVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHS TGGMDELYKSGLRSRSAAAGAGGAARAALGVQVETISPGDGRTFP KRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWE EGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLE SAGGSAGGSAGGSAGGSAGGPRAQASNSMATESILHFAGYNKQN TTLGATQLSERPACVKKDYSNFMASLNLRNRYAGEVYGMIRFSGT TGQMSDLNKMMVQDLHSALDRSHPQHYTQAMFKLTAMLISSKDC DPQLLHHLCWGPLRMFNEHGMETALACWEWLLAGKDGVEVPFM REMAGAWHMTVEQKFGLFSAEIKEADPLAASEASQPKPCPPEVTP HYIWIDFLVQRFEIAKYCSSDQVEIFSSLLQRSMSLNIGGAKGSMNR HVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSCP PKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQDTR SNLDITVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQ LHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGENSVAN WRSKYISLSEKQWKDNVNLAWSISPYLAVQLPARFKNTEAIGNEVT RLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPTDPPT GLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKM GYVREYILWAASKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLL DQLVEEITGSLSGPAKDFYQREFDFFNKITNVSAIIKPYPKGDERKK ACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLA KFKVKRCGVSELEKEGLRCRSDSEDECSTQEADGQKISWQAAIFK VGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIE CIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRS MAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGDNLG WEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDA VVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKVIQS CFLSNRSRTYDMIQYYQNDIPY* |
| FKBP-PIP5K | pTagBFP-FKBP1A3-108-PIP5K1C1-635, D101R, R304D, R445E, K446E | This Study |
MVSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWE RVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKT LGWEAFTETLYPADGGLEGRNDMALKLVGGSHLIANAKTTYRSKK PAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPSK LGHKLNSGLRSRSAAAGAGGAARAALGVQVETISPGDGRTFPKRG QTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGV AQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLESAG GSAGGSAGGSAGGSAGGPRAQASNSAVDLQAMELEVPDEAESA EAGAVPSEAAWAAESGAAAGLAQKKAAPTEVLSMTAQPGPGHGK KLGHRGVDASGETTYKKTTSSTLKGAIQLGIGYTVGHLSSKPER[R] VLMQDFYVVESIFFPSEGSNLTPAHHFQDFRFKTYAPVAFRYFREL FGIRPDDYLYSLCNEPLIELSNPGASGSLFYVTSDDEFIIKTVMHKEA EFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQSGGKNIRVVVMNNI LPRVVKMHLKFDLKGSTYKRRASKKEKEKSFPTYKDLDFMQDMPE GLLLDADTFSALVKTLQ[D]DCLVLESFKIMDYSLLLGVHNIDQHERE RQAQGAQSTSDEKRPVGQKALYSTAMESIQGGAARGEAIESDDT MGGIPAVNGRGERLLLHIGIIDILQSYRFIKKLEHTWKALVHDGDTVS VHRPSFYAERFFKFMSNTVF[EE]NSSLKSSPSKKGRGGALLAVKPL GPTAAFSASQIPSEREEAQYDLRGARSYPTLEDEGRPDLLPCTPPS FEEATTASIATTLSSTSLSIPERSPSETSEQPRYRRRTQSSGQDGR PQEEPPAEEDLQQITVQVEPACSVEIVVPKEEDAGVEASPAGASAA VEVETASQASDEEGAPASQASDEEDAPATDIYF* |
| EGFP-PH-PLCd1 | PLCd11-170-EGFP | (Várnai and Balla, 1998) | MDSGRDFLTLHGLQDDEDLQALLKGSQLLKVKSSSWRRERFYKLQ EDCKTIWQESRKVMRTPESQLFSIEDIQEVRMGHRTEGLEKFARD VPEDRCFSIVFKDQRNTLDLIAPSPADAQHWVLGLHKIIHHSGSMD QRQKLQHWIHSCLRKADKNKDNKMSFKELQNFLKDPPVATMVSK GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYV QERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHK LEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNT PIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLG MDELYK* |
| FKBP-PI-PLC | B. cereus PI-PLC32-329, W78A, W273A-FKBP1A3-108-TagBFP2 | (Pemberton et al., 2020) | MASSVNELENWSKWMQPIPDNIPLARISIPGTHDSGTFKLQNPIKQ V[A]GMTQEYDFRYQMDHGARIFDIRGRLTDDNTIVLHHGPLYLYVT LHEFINEAKQFLRDNPSETIIMSLKKEYEDMKGAEDSFSSTFEKNYF VDPIFLKTEGNIKLGDARGKIVLLKRYSGSNESGGYNNFYWPDNET FTTTVNQNVNVTVQDKYKVSYDEKVKSIKDTMNETMNNSEDLNHL YINFTSLSSGGTA[A]NSPYYYASYINPEIANHIKQKNPARVGWVIQD YINEKWSPLLYQEVIRANKSLIKERILQSTVPMGVQVETISPGDGRT FPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRG WEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL KLERDPPVATMVSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKP YEGTQTMRIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFK QSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFT SNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLVGGSHLIA NAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVA VARYCDLPSKLGHKLNSGLRSRSAAATLDHNQPYHICRGFTCFKK PPTPPPEPET* |
| FKBP-DGKα | mRFP-FKBP1A3-108-DGKA isoform b394-773 | This Study |
MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGF KWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPV MQKKTMGWEASTERMYPEDGALKGEIKMRLKLKDGGHYDAEVKT TYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHSTGA SGLRSRSAAAGAGGAARAALGVQVETISPGDGRTFPKRGQTCVV HYTGMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSV GQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELLKLESAGGSAGG SAGGSAGGSAGGPRAQASRSDDLNLSTSEALRIDPVPNTHPLLVF VNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRLFKDVP DSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLR WGGGYEGQNLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPV PFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFAT SESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNL WGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVV GLEGAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWM QTPCTIKITHKNQMPMLMGPPPRSTNFFGFLS* |
| M3 | pcDNA3.1-HAx3-AchR-CHRM32-590 | J. Wess | MYPYDVPDYAYPYDVPDYAYPYDVPDYADTLHNNSTTSPLFPNISS SWIHSPSDAGLPPGTVTHFGSYNVSRAAGNFSSPDGTTDDPLGGH TVWQVVFIAFLTGILALVTIIGNILVIVSFKVNKQLKTVNNYFLLSLACA DLIIGVISMNLFTTYIIMNRWALGNLACDLWLAIDYVASNASVMNLLV ISFDRYFSITRPLTYRAKRTTKRAGVMIGLAWVISFVLWAPAILFWQ YFVGKRTVPPGECFIQFLSEPTITFGTAIAAFYMPVTIMTILYWRIYK ETEKRTKELAGLQASGTEAETENFVHPTGSSRSCSSYELQQQSMK RSNRRKYGRCHFWFTTKSWKPSSEQMDQDHSSSDSWNNNDAAA SLENSASSDEEDIGSETRAIYSIVLKLPGHSTILNSTKLPSSDNLQVP EEELGMVDLERKADKLQAQKSVDDGGSFPKSFSKLPIQLESAVDT AKTSDVNSSVGKSTATLPLSFKEATLAKRFALKTRSQITKRKRMSLV KEKKAAQTLSAILLAFIITWTPYNIMVLVNTFCDSCIPKTFWNLGYWL CYINSTVNPVCYALCNKTFRTTFKMLLLCQCDKKKRRKQQYQQRQ SVIFHKRAPEQAL* |
| GFP-Nir2 | EGFP-PITPNM1v2 | (Kim et al., 2015) |
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMP EGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNI LGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHY QQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAA GITLGMDELYKSGLRSRAQASNSMLIKEYHILLPMSLDEYQVAQLY MIQKKSREESSGEGSGVEILANRPYTDGPGGSGQYTHKVYHVGSH IPGWFRALLPKAALQVEEESWNAYPYTRTRYTCPFVEKFSIEIETYY LPDGGQQPNVFNLSGAERRQRILDTIDIVRDAVAPGEYKAEEDPRL YHSVKTGRGPLSDDWARTAAQTGPLMCAYKLCKVEFRYWGMQA KIEQFIHDVGLRRVMLRAHRQAWCWQDEWTELSMADIRALEEETA RMLAQRMAKCNTGSEGSEAQPPGKPSTEARSAASNTGTPDGPEA PPGPDASPDASFGKQWSSSSRSSYSSQHGGAVSPQSLSEWRMQ NIARDSENSSEEEFFDAHEGFSDSEEVFPKEMTKWNSNDFIDAFAS PVEAEGTPEPGAEAAKGIEDGAQAPRDSEGLDGAGELGAEACAVH ALFLILHSGNILDSGPGDANSKQADVQTLSSAFEAVTRIHFPEALGH VALRLVPCPPICAAAYALVSNLSPYSHDGDSLSRSQDHIPLAALPLL ATSSSRYQGAVATVIARTNQAYSAFLRSPEGAGFCGQVALIGDGV GGILGFDALCHSANAGTGSRGSSRRGSMNNELLSPEFGPVRDPLA DGVEGLGRGSPEPSALPPQRIPSDMASPEPEGSQNSLQAAPATTS SWEPRRASTAFCPPAASSEAPDGPSSTARLDFKVSGFFLFGSPLG LVLALRKTVMPALEAQMRPACEQIYNLFHAADPCASRLEPLLAPKF QAIAPLTVPRYQKFPLGDGSSLLLADTLQTHSSLFLEELEMLVPSTP TSTSGAFWKGSELATDPPAQPAAPSTTSEVVKILERWWGTKRIDY SLYCPEALTAFPTVTLPHLFHASYWESADVVAFILRQVIEKERPQLA ECEEPSIYSPAFPREKWQRKRTQVKIRNVTSNHRASDTVVCEGRP QVLSGRFMYGPLDVVTLTGEKVDVYIMTQPLSGKWIHFGTEVTNS SGRLTFPVPPERALGIGVYPVRMVVRGDHTYAECCLTVVARGTEA VVFSIDGSFTASVSIMGSDPKVRAGAVDVVRHWQDSGYLIVYVTG RPDMQKHRVVAWLSQHNFPHGVVSFCDGLTHDPLRQKAMFLQSL VQEVELNIVAGYGSPKDVAVYAALGLSPSQTYIVGRAVRKLQAQCQ FLSDGYVAHLGQLEAGSHSHASSGPPRAALGKSSYGVAAPVDFLR KQSQLLRSRGPSQAEREGPGTPPTTLARGKARSISLKLDSEE* |
| iRFP-Nir1-LNS2 | miRFP670-Nir1613-897 | This Study |
MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVA VRCRIGNPSTEYCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERI RTAGSLRALCDDTVLLFQQCTGYDRVMVYRFDEQGHGLVFSECH VPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVTYQPVPLEP RLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGG KLWGLVVCHHYLPRFIRFELRAICKRLAERIATRITALESGGSGGMP ANPREKWLRKRTQVKLRNVTANHRANDVIAAEDGPQVLVGRFMY GPLDMVALTGEKVDILVMAEPSSGRWVHLDTEITNSSGRITYNVPR PRRLGVGVYPVKMVVRGDQTCAMSYLTVLPRGMECVVFSIDGSFA ASVSIMGSDPKVRPGAVDVVRHWQDLGYMILYITGRPDMQKQRVV SWLSQHNFPQGMIFFSDGLVHDPLRQKAIFLRNLMQECFIKISAAY GSTKDISVYSVLGLPASQIFIVGRPTKKYQTQCQFLSEGYAAHLAAL EASHRSRPKKN* |
| iRFP-Tubby | M. musculus Tubby243-505-miRFP670 | (Quinn et al., 2008) | MVDIEVQDLEEFALRPAPQGITIKCRITRDKKGMDRGMYPTYFLHL DREDGKKVFLLAGRKRKKSKTSNYLISVDPTDLSRGGDSYIGKLRS NLMGTKFTVYDNGVNPQKASSSTLESGTLRQELAAVCYETNVLGF KGPRKMSVIVPGMNMVHERVCIRPRNEHETLLARWQNKNTESIIEL QNKTPVWNDDTQSYVLNFHGRVTQASVKNFQIIHGNDPDYIVMQF GRVAEDVFTMDYNYPLCALQAFAIALSSFDSKLACETVPRARDPPV ATMVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEH DHRVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMP VAVRCRIGNPSTEYCGLMHRPPEGGLIIELERAGPSIDLSGTLAPAL ERIRTAGSLRALCDDTVLLFQQCTGYDRVMVYRFDEQGHGLVFSE CHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVTYQPVP LEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLV VGGKLWGLVVCHHYLPRFIRFELRAICKRLAERIATRITALES* |
| iRFP-PH-PLCδ1 | iRFP713-R. norvegicus PLCD12-131 |
(Idevall-Hagren et al., 2012) |
MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGSDN LPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGF TMRKDAGFIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRR LQAAETLESACAAAAQEVRKITGFDRVMIYRFASDFSGEVIAEDRC AEVESKLGLHYPASTVPAQARRLYTINPVRIIPDINYRPVPVTPDLNP VTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGLIV CHHRTPYYVDLDGRQACELVAQVLAWQIGVMEECTRDLELKLHGL QDDPDLQALLKGSQLLKVKSSSWRRERFYKLQEDCKTIWQESRKV MRSPESQLFSIEDIQEVRMGHRTEGLEKFARDIPEDRCFSIVFKDQ RNTLDLIAPSPADAQHWVQGLRKIIHHSGSMDQRQK* |
| TagBFP2-CAAX | TagBFP2-HRAS172-189 | (Goulden et al., 2019) |
MVSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWE RVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKT LGWEAFTETLYPADGGLEGRNDMALKLVGGSHLIANAKTTYRSKK PAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPSK LGHKLNSGLRSRAQASNSAVDNPPDESGPGCMSCKCVLS* |