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[Preprint]. 2024 Feb 29:rs.3.rs-3964789. [Version 1] doi: 10.21203/rs.3.rs-3964789/v1

Figure 1.

Figure 1

Morris Water Maze to evaluate the effect of genetic Fshr depletion in 5–month–old 3xTg females on acquisition and retrieval of spatial memory. (A) In 5–month–old 3xTg female mice, latency to find the hidden platform (seconds, s) was significantly shorter for 3xTg;Fshr−/− on days 3, 4 and 5 of the acquisition trials compared with 3xTg;Fshr+/+ mice. The effect was also significant on day 5 in 3xTg;Fshr+/− mice compared with 3xTg;Fshr+/+ mice. Both time courses and area under the curve (AUC, days 1 to 5) are shown. Mice that showed repeated episodes of extensive floating (>10 s per trial or >25% of trial time across 5 days) were excluded from the analysis (B) The effect of Fshr depletion in the same mouse groups at day 7 in the retention trial which platform was removed and time spent around the 40–cm platform center point (platform zone) was determined. Mice that showed repeated episodes of extensive oating (>25% of trial time) were excluded from the analysis. N=8 to 13 mice per group; Mean ± SEM; P values as shown, significant at P≤0.05; repeated measures ANOVA followed by Fisher’s Least Significant Difference post–hoc analyses).