Table 2.
Comparison of PCR and AmpliSeq assay results for pfhrp2/3 genotyping
| pfhrp2 | AmpliSeq | ||||
|---|---|---|---|---|---|
| Deletion | Presence | Inconclusive | Total | ||
| PCR | Deletion | 33 | 1 | 7 | 41 (49.4%) |
| Presence | 18 | 0 | 24 | 42 (51.6%) | |
| Total | 51 (61.4%) | 1 (1.2%) | 31 (37.3%) | 83 (100%) | |
| pfhrp3 | AmpliSeq | ||||
| Deletion | Presence | Inconclusive | Total | ||
| PCR | Deletion | 10 | 13 | 4 | 27 (32.5%) |
| Presence | 1 | 55 | 0 | 56 (67.5%) | |
| Total | 11 (13.3%) | 68 (81.9%) | 4 (4.8%) | 83 (100%) | |
Using the PCR data for pfhrp2/3 genotyping, which was able to genotype all samples, parasites from NJ Clusters 1 and 2 were predominantly genotyped as pfhrp2+ / pfhrp3+ (87% and 78% respectively), while pfhrp2− / pfhrp3 + was common in Cluster 3 (78%; Fig. 7, Supplementary Table S7). Parasites with both genes present were frequent in Santa Emilia (83%) and Andoas (50%). However, all parasites from Mazan carried the double deletion of both genes.