Table 2.
List of antibodies used for flow cytometry.
| Cell types | Target | Fluorophore | Concentration / Dilution | Source and Catalog # |
|---|---|---|---|---|
| Live / dead | 7-AAD | 7-AAD | 1:60 | BD Biosci. 51-68981e |
| Leukocytes | CD45 (clone 30-F11) | Per-CP 5.5 | 0.2 mg/mL | Biolegend 103131 |
| CD45.2 (IV) (clone 104) | BUV737 | 0.2 mg/mL | BD Biosci. 612778 | |
| T cells | CD4 | APC-Cy7 | 0.2 mg/mL | Biolegend 100413 |
| CD8a | BV510 | 0.05 mg/mL | Biolegend 100751 | |
| B cells | CD19 | BUV395 | 0.2 mg/mL | BD Biosci. 563557 |
| Dendritic cells | CD11 b | PE | 0.2 mg/mL | Biolegend 101207 |
| CD11c | APC | 0.2 mg/mL | Invitrogen 17-0114-81 | |
| CD103 (clone 2E7) | PE-Cy7 | 0.2 mg/mL | Biolegend 121425 | |
| Ly6c | Efluor450 | 0.2 mg/mL | Invitrogen 48-5932-80/2 | |
| Dump gate | CD64 | FITC | 0.5 mg/mL | Biolegend 161007 |
| Ly6g | FITC | 0.5 mg/mL | Biolegend 127606 | |
| Siglec F | FITC | 0.5 mg/mL | Biolegend 155503 |