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[Preprint]. 2024 Feb 26:rs.3.rs-3976896. [Version 1] doi: 10.21203/rs.3.rs-3976896/v1

Figure 2. Early loss of cortical TH+ projection in 5xFAD mice.

Figure 2

a Representative 20x confocal images showing neuritic plaques (Methoxy-X04, blue), putative NE projections (TH, green), microglia (Iba1, red), and astrocytes (GFAP, magenta) in the ACC at 4 months (scale bar = 200μm). Analyses were performed in ACC (b-e) and V1 (f-i). b, f Quantification of senile plaque load labeled with Methoxy-X04. c, g, d, h Area fraction of both microglia (c, g) and astrocyte (d, h) increased early in 5xFAD mice. e, i Decrease in TH+ projections in ACC at 4 months (e) and in V1 at 6 months (i) in 5xFAD mice compared to littermate controls. n = 7–9 mice per genotype per age group. One-way ANOVA with Bonferroni correction (b, f) or two-way ANOVA comparing between genotypes and ages with Bonferroni correction for genotype comparisons within the same age group (c-e, g-i); *p<0.05, **p<0.01, ***p<0.001.