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[Preprint]. 2024 Feb 26:rs.3.rs-3976896. [Version 1] doi: 10.21203/rs.3.rs-3976896/v1

Figure 4. Loss of microglial homeostatic signature and β2AR expression is dependent on age and amyloid pathology in 5xFAD mice.

Figure 4

a Experimental paradigm for microglia isolation and analysis. b-c Expression of microglial homeostatic marker P2RY12 (b) and TMEM119 (c) is downregulated early in plaque-associated microglia and progressively decreases in plaque-distal microglia with aging (n = 7–9 mice per genotype per age group). d β2AR mRNA levels were reduced in both plaque-associated and plaque-distal microglia (n = 3–5 mice per genotype per age group). Cortices from different age groups were collected at different times, and thus, analyzed independently and normalized to the age-matched controls. One-way ANOVA with Bonferroni post-hoc correction; *p<0.05, **p<0.01, ***p<0.001.