Table 2.
Bacteria | ||
---|---|---|
Name | Relevant feature | Reference |
PA01 | 83 | |
PADR6 | Frame shift mutation in wzy | 11 |
PAER5b | Nonsense mutation in wapH | 11 |
PAER6b | Frame shift mutation in galU | 11 |
PAER10b | Nonsense mutation in algC | 11 |
Bacteriophages | ||
Name | Relevant featuresa | Reference |
DEV | 8 | |
DEV Δ53 | Deletion 29656–32966; it eliminates gp53 | This work |
Plasmids | ||
Name | Relevant featuresa | Reference |
pCas3cRh | It expresses the components of Type l-C CRISPR-Cas system | 31 |
pCas3-01 | pCas3cRh derivative carrying DEV 31196–31229 region; it expresses cr-RNA53 targeting gp53 | This work |
pCas3-09 | pCas3-01 derivative carrying the two gp53 flanking regions with coordinates 29171 −29655 and 32967–33450 | This work |
pD53 | pGM2148 derivative with 9 silent point mutations in the 31198-31230 region targeted by cr-RNA53 | This work |
pGM931 | Shuttle vector carrying araC-araBp region | 61 |
pGM2148 | pGM931 derivative carrying the gp53-encoding 29656–32949 region | This work |
gp72_pET30b(+) | untagged gp72 cloned in pET30b(+) between Ndel and Xhol | This work |
gp73_pET22b(+) | gp73 cloned in pET22b(+) between Ndel and Xhol comprising C-terminal 6x his tag | This work |
Coordinates refer to Genbank MF490238.1