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[Preprint]. 2024 Feb 21:rs.3.rs-3941185. [Version 1] doi: 10.21203/rs.3.rs-3941185/v1

Table 2.

Bacteria, phages and plasmids

Bacteria
Name Relevant feature Reference
PA01 83
PADR6 Frame shift mutation in wzy 11
PAER5b Nonsense mutation in wapH 11
PAER6b Frame shift mutation in galU 11
PAER10b Nonsense mutation in algC 11
Bacteriophages
Name Relevant featuresa Reference
DEV 8
DEV Δ53 Deletion 29656–32966; it eliminates gp53 This work
Plasmids
Name Relevant featuresa Reference
pCas3cRh It expresses the components of Type l-C CRISPR-Cas system 31
pCas3-01 pCas3cRh derivative carrying DEV 31196–31229 region; it expresses cr-RNA53 targeting gp53 This work
pCas3-09 pCas3-01 derivative carrying the two gp53 flanking regions with coordinates 29171 −29655 and 32967–33450 This work
pD53 pGM2148 derivative with 9 silent point mutations in the 31198-31230 region targeted by cr-RNA53 This work
pGM931 Shuttle vector carrying araC-araBp region 61
pGM2148 pGM931 derivative carrying the gp53-encoding 29656–32949 region This work
gp72_pET30b(+) untagged gp72 cloned in pET30b(+) between Ndel and Xhol This work
gp73_pET22b(+) gp73 cloned in pET22b(+) between Ndel and Xhol comprising C-terminal 6x his tag This work
a

Coordinates refer to Genbank MF490238.1