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Determination of the LD50 of nanosilver in mice (n = 5).
| Dose in mg/kg B.W | No. of dead animals in each group | A | B | A × B | Σ (A × B) | Dm | Calculated Ld50 |
|---|---|---|---|---|---|---|---|
| 100 | 0/5 | 0 | 50 | 0 | 425 | 300 |
Dm−Σ (A × B)/N 300–425/5 = 215 mg/kg B.W |
| 150 | 1/5 | 0.5 | 50 | 25 | |||
| 200 | 2/5 | 1.5 | 50 | 75 | |||
| 250 | 3/5 | 2.5 | 50 | 125 | |||
| 300 | 5/5 | 4 | 50 | 200 |
DM, the highest dose that kills all inoculated mice; A, mean of dead mice between 2 successive groups; B, constant factor between 2 successive doses; N, number of mice per group. Σ = Sum of (A × B).
