Table 2.
Diagnostic variables (n=24; 23 peptides and BMI*) selected by random forest (RF).
| Peptide | Protein (Gene) Name | RF importance score | WMW P value | WMW odds (95% CI) | Function | Peptide Sequence | Accession Number |
|---|---|---|---|---|---|---|---|
| ACTG(96–113) | Actin, cytoplasmic 2 (ACTG1) | 1 | 1.85e-13 | 3.12 (2.28,4.23) | Cell motility | VAPEEHPVLLTEAPLNPK | P63261–1 |
| HEMO(198–201) | Hemopexin (HPX) | 1 | 3.02e-10 | 0.39 (0.29,0.53) | Acute phase protein, transports heme to liver for breakdown | YYCFQGNQFLR | P02790–1 |
| KNG1(317–324) | Kininogen-1 (KNG1) | 1 | 3.33e-10 | 0.39 (0.29,0.53) | Coagulation factor and regulator of Insulin-like Growth Factor | YFIDFVAR | P01042–1 |
| CO5(767–782) | Complement C5 (C5) | 1 | 2.02e-09 | 0.41 (0.31,0.55) | Complement component | ALEQDLPVNIK | P01031–1 |
| C1R(229–235) | Complement C1r subcomponent (C1R) | 1 | 4.28e-09 | 0.42 (0.31,0.56) | Serine protease, part of the first component of the classical pathway of the complement system | GLTLHLK | P00736–1 |
| A1BG(262–280) | Alpha-1B-glycoprotein (A1BG) | 1 | 9.43e-09 | 0.49 (0.37,0.65) | Novel member of immunoglobulin superfamily | IFFHLNAVALGDGGHYTCR | PO4217–1 |
| CD14(94–111) | CD14 Monocyte differentiation antigen (CD14) | 1 | 6.67e-07 | 0.48 (0.36,0.65) | Coreceptor for bacterial lipopolysaccharide | LTVGAAQVPAQLLVGALR | P08571–1 |
| HPT(392–401) | Haptoglobin (HP) | 1 | 3.70e-05 | 0.55 (0.42,0.73) | Antioxidant, has antibacterial activity, and plays a role in modulating many aspects of the acute phase response | VTSIQDWVQK | P00738–1 |
| HRG(188–201) | Histidine-rich glycoprotein (HRG) | 1 | 0.0002 | 0.59 (0.44,0.78) | Regulates many processes such as immune complex and pathogen clearance, cell chemotaxis, cell adhesion, angiogenesis, coagulation and fibrinolysis | GGEGTGYFVDFSVR | P04196–1 |
| CNDP1(182–192) | Beta-Ala-His dipeptidase (CNDP1) | 1 | 0.0009 | 1.60 (1.21,2.11) | Catalyzes the peptide bond hydrolysis in Xaa-His dipeptides | ALEQDLPVNIK | Q96KN2–1 |
| CRAC1(170–178) | Cartilage acidic protein 1 (CRTAC1) | 1 | 0.02 | 1.39 (1.05,1.82) | Glycosylated extracellular matrix protein | GVASLFAGR | Q9NQ79–1 |
| KNG1(479–496) | Kininogen-1 (KNG1) | 1 | 0.09 | 0.79 (0.60,1.04) | See above | LDDDLEHQGGHVLDHGHK | P01042–1 |
| HPT(216–227) | HP | 0.999 | 2.31e-05 | 0.54 (0.41,0.72) | See above | DIAPTLTLYVGK | P00738–1 |
| HEMO(92–102) | HPX | 0.999 | 0.0002 | 0.58 (0.44,0.77) | See above | NFPSPVDAAFR | P02790–1 |
| IC1(287–298) | Plasma protease C1 inhibitor (SERPING1) | 0.998 | 4.99e-06 | 0.52 (0.39,0.69) | Plays crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins | LVLLNAIYLSAK | P05155–1 |
| CRAC1(101–108) | Cartilage acidic protein 1 (CRTAC1) | 0.998 | 0.10 | 1.26 (0.96,1.66) | Glycosylated extracellular matrix protein | SSPYYALR | Q9NQ79–1 |
| MASP1(194–208) | Mannan-binding lectin serine protease 1 (MASP1) | 0.983 | 3.00e-05 | 0.55 (0.41,0.73) | Functions in lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them | TGVITSPDFPNPYPK | P48740–1 |
| CFAI(346–358) | Complement factor I (CFI) | 0.964 | 3.08e-06 | 0.51 (0.38,0.68) | Trypsin-like serine protease that plays an essential role in regulating the immune response by controlling all complement pathways | AQLGDLPWQVAIK | P05156–1 |
| VTDB(95–114) | Vitamin D-binding protein (GC) | 0.955 | 0.007 | 0.68 (0.52,0.90) | Vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation | SCESNSPFPVHPGTAECCTK | P02774–1 |
| FA5(1506–1517) | Coagulation factor V (F5) | 0.943 | 3.54e-06 | 0.51 (0.38,0.68) | Central regulator of hemostasis.; serves as critical cofactor for the prothrombinase activity of factor Xa that results in activation of prothrombin to thrombin. | EFNPLVIVGLSK | P12259–1 |
| HABP2(123–135)* | Hyaluronan-binding protein 2 (HABP2) | 0.937 | 0.65 | 0.94 (0.71,1.23) | Extracellular serine protease that binds hyaluronic acid, involved in extrinsic pathway of blood coagulation, activates urinary plasminogen activator and coagulation factor VII | GQCLITQSPPYYR | Q14520–1 |
| VTDB(208–218) | GC | 0.934 | 5.47e-06 | 0.52 (0.39,0.70) | See above | HLSLLTTLSNR | P02774–1 |
| AMBP(298–309) | Alpha-1-microglobulin (AMBP) | 0.906 | 5.06e-06 | 0.52 (0.39,0.69) | Antioxidant and tissue repair protein | AFIQLWAFDAVK | P02760–1 |
*
Results for BMI: RF importance score 0.995; WMW p value 8.3e-06; WMW odds (95% CI) 1.91 (1.44, 2.54) Amino Acid Number (per Swiss UniProt db searched 4.8.21); Table ordered according to RF score then WMW p value; WMW Odds: >1 indicates higher probability that a randomly chosen OA value will be greater than a randomly chosen control value, <1 indicates higher probability that a randomly chosen OA value will be lesser than a randomly chosen control value. The finally selected 17 uncorrelated peptides are underlined.
The finally selected 8 significant peptides are in bold;