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. Author manuscript; available in PMC: 2024 Mar 11.
Published in final edited form as: Cell Rep. 2024 Jan 5;43(1):113636. doi: 10.1016/j.celrep.2023.113636

Figure 1. Ultrasensitive single-cell proteomic mapping of ALS motor neurons.

Figure 1.

(A) Proteins (high-confidence master proteins, HCMPs; 5% global FDR) identified across single MNs (n = 6/donor) laser excised from ALS or CTL donor tissues (n = 3/diagnosis).

(B) Principal-component analysis (PCA) dimensionality reduction of the top 500 most-variable proteins measured across ALS and CTL MNs.

(C) Differential protein abundance in ALS vs. CTL MNs with correction for donor origin. Significant DAPs (|log2(ALS/CTL)| ≥ 1.5 ∩ p-adj < 0.05) indicated in purple.

(D) (Left) Age and PMI distributions for ALS and CTL donors; median ± range. (Right) Differential protein abundance with correction for additional covariates (age, sex, PMI). Significant DAPs indicated in purple.

(E) PPI network of significant DAPs. Node color indicates fold change in normalized protein abundance; edges denote high-confidence PPIs.