Fig. 4.

Palbociclib inhibits the activation of the AIM2 inflammasomes but not NLRP3 inflammasomes in A549 cells after radiation. (a–c) Activation of AIM2 and NLRP3 inflammasomes detected using CO-IP (a) and quantification graphs (b, c) after palbociclib treatment. (d, e) After palbociclib treatment, AIM2 specks were detected through immunofluorescence staining (d), and quantification graphs are shown (e; mean ± SD, one-way ANOVA with Tukey's multiple comparison test; third vs. fourth group p < 0.001, n = 3 independent experiments; scale bar: 100 μm). (f, g) After palbociclib treatment, NLRP3 specks were detected using immunofluorescence staining (f), and quantification graphs are shown (g; mean ± SD, one-way ANOVA with Tukey's multiple comparison test; third vs. fourth group p = 0.8351, n = 3 independent experiments). Scale bar: 100 μm.