(a) Release
kinetics of calcein from 1:1 PG/PE liposomes (200 μM)
as a function of time. At t = 50 s, either peptide
(at a final concentration of 10 μM) or Triton-X100 (final concentration
of 10% w/v) was added to each well and calcein release was measured
with an excitation wavelength of 500 nm and an emission wavelength
of 520 nm. (b) Percent release of calcein from 1:1 PG/PE liposomes
(200 μM) as a function of peptide concentration. The 100% release
level was determined using Triton-X detergent. Curves are the mean
of three independent experiments with standard deviation error bars
for WT (closed squares), I4R (open circles), L7R (closed triangles),
V8R (open triangles), V8R (open triangles), F15R (closed inverted
triangles), I23R (open squares), F26R (pluses), and L40R (crosses).