Table 2. Functional Properties of Enterocin Peptides.
| peptide | MICa (μM) | initial calcein release rateb (dFo/dt)c | percent calcein released at MIC (%) |
|---|---|---|---|
| WT | 10.6 ± 0.1 | 9.2 ± 1.1 | 30.5 ± 1.3 |
| I4R | 6.1 ± 0.2 | 18.9 ± 1.4 | 24.0 ± 1.5 |
| L7R | 22.2 ± 0.1 | 6.4 ± 0.8 | 27.7 ± 1.2 |
| V8R | 11.5 ± 0.1 | 8.2 ± 0.5 | 30.1 ± 1.1 |
| F15R | 10.9 ± 0.1 | 9.1 ± 0.9 | 33.4 ± 1.3 |
| I23R | 3.1 ± 0.1 | 19.8 ± 0.7 | 24.5 ± 1.0 |
| F26R | 10.5 ± 0.1 | 9.9 ± 1.0 | 26.1 ± 1.5 |
| L40R | 6.4 ± 0.2 | 18.1 ± 1.2 | 25.6 ± 1.4 |
a
MIC.
b
Calcein release rate from 1:1 PG/PE liposomes at 25 °C.
c
dFo/dt is the time derivative of the emission intensity at 520 nm and t = 0.
d
%calcein release is relative to Trion X-100 plateau (which measures 100% release).