Table 3. Comparison of Measured Observables among the WT 2M60 and 7 Mutants^e.

rmsda	CDb	Tmc	MICd	PEPt	PEP0f	[ΔCAL]g	k[CAL]h	Wi
1.I23R	1.WT	1.I4R	1.I4R	1.I23R	1.I4R	1.F15R	1.I4R	1.I23R
1.L7R	2.I4R	1.I23R	1.I23R	2.I4R	1.I23R	1.I23R	1.I23R	2.I4R
1.I4R	2.L7R	1.L40R	1.L40R	2.L40R	1.L40R	1.L40R	1.L40R	2.L40R
4.L40R	2.V8R	4.WT	4.WT	4.WT	4.WT	4.WT	4.WT	4.WT
5.F26R	2.F26R	4.V8R	4.V8R	4.V8R	4.V8R	4.I4R	4.V8R	4.V8R
5.F15R	6.F15R	4.F15R	4.F15R	4.F15R	4.F15R	4.V8R	4.F15R	4.F15R
7.V8R	6.I23R	7.F26R	4.F26R	4.F26R	4.F26R	4.F26R	4.F26R	4.F26R
	6.L40R	7.L7R	8.L7R	8.L7R	8.L7R	8.L7R	8.L7R	8.L7R

^armsd (Å), from highest to lowest.

^bNear UV CD signal magnitude, from more to less signal.

^cMelting temperature, from lowest to highest.

^dMIC value, from better to worse.

^ePeptide added to growing bacterial culture, from more to less effective.

^fPeptide added at the start of a bacterial culture, from more to less effective.

^gCalcein release, from more to less release.

^hCalcein release rate, from fast to slow release.

ⁱWork at r_ee = 60 Å, from low to high work value.