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. 2024 Feb 15;27(3):109219. doi: 10.1016/j.isci.2024.109219

Figure 1.

Figure 1

Ferroptosis is more prevalent in cardiomyocytes than apoptosis and necroptosis in infarcted postnatal hearts

LAD-O was performed at either P1 or P7, heart tissue section prepared at 1, 3, or 6 DPMI.

(A–G) Infarct zone stained for cCasp3 (magenta), MF20 (green), and DAPI (blue). Arrow: cardiomyocytes positive for cCasp3, ratio quantified in (G). Arrowhead: non-cardiomyocyte positive for cCasp3.

(H–N) Infarct zone stained for pMLKL (magenta), cTnT (green), and DAPI (blue). Arrow: cardiomyocytes positive for pMLKL, ratio quantified in (N). Arrowhead: non-cardiomyocyte positive for pMLKL.

(O–U) Infarct zone stained for Ptgs2 (magenta), cTnT (green), and DAPI (blue). Arrow: cardiomyocytes positive for Ptgs2, ratio quantified in (U). All bar graphs represent mean ± SD. ∗p < 0.05; NS, not significant by t test. NS in (G), no significance among all groups. #, infarct zone. Scale bar, 25 μm (A–F, H–M, O–T). See also Figures S1 and S2.