Skip to main content
. 2024 Feb 15;27(3):109219. doi: 10.1016/j.isci.2024.109219
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Myosin, sarcomere (MHC) antibody Developmental Studies Hybridoma Bank Cat# MF 20; RRID: AB_2147781
Cleaved Caspase 3 Cell Signaling Technology Cat# 9664; RRID: AB_2070042
4-Hydroxynonenal Bioss Cat# bs-6313R; RRID: AB_2827741
Phospho-MLKL (Ser345) Cell Signaling Technology Cat# 37333; RRID: AB_2799112
Cox2/Cyclooxygenase 2/PTGS2 Proteintech Cat# 12375-1-AP; RRID: AB_2085127
Anti-Troponin T, Cardiac Isoform Ab-1 Monoclonal Antibody, Unconjugated Lab Vision Cat# MS-295-P1ABX; RRID:AB_61809
Monoclonal Anti-Actin, alpha-Smooth Muscle – Cy3™ Sigma-Aldrich Cat# C6198; RRID: AB_476856
Recombinant Anti-pSmad3 (phosphor S423 + S425) Abcam Cat# ab52903; RRID: AB_882596
Anti-pSmad2 (phospho S255) Abcam Cat#ab188334;
RRID: AB_2732791
Anti-TGF beta Receptor II Abcam Cat# ab186838;
RRID: AB_2728775
Ferritin Light Chain Antibody Abcam Cat# ab109373;
RRID: AB_10862715
Anti-Connexin 45/GJA7/Cx45 Antibody Abcam Cat# ab135474;
RRID: AB_2753195
Anti-Human/Rat Connexin-43 Antibody Sigma-Aldrich Cat# C6219;
RRID: AB_476857
Mouse PDGF R alpha Antibody R and D Systems Cat# AF1062;
RRID: AB_2236897
Vimentin antibody Abcam Cat# ab92547;
RRID: AB_10562134
Thrombospondin antibody Abcam Cat# ab85762;
RRID: AB_10674322
Ferritin Heavy Chain (B-12) Santa Cruz Biotechnology Cat# sc-376594;
RRID: AB_11150859
Monoclonal Anti-alpha-Tubulin antibody produced in mouse Sigma-Aldrich Cat# T5168;
RRID: AB_477579
CD181 (CXCR1) Monoclonal Antibody Thermo Fisher Scientific Cat# 12-1819-42;
RRID: AB_2848258
FITC anti-mouse CD182 (CXCR2) BioLegend Cat# 149310;
RRID:AB_2566148
CXCR2 Polyclonal Antibody Thermo Fisher Scientific Cat# PA5-83213;
RRID: AB_2790369
Cardiac Troponin T Monoclonal Antibody Thermo Fisher Scientific Cat# MA5-12960;
RRID: AB_11000742
GPX4 antibody Proteintech Cat# 67763-1-Ig;
RRID: AB_2909469
ACSL4 Polyclonal Antibody Thermo Fisher Scientific Cat# PA5-27137;
RRID: AB_2544613
AMID Polyclonal Antibody Thermo Fisher Scientific Cat# PA5-103183;
RRID: AB_2852551
Goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody Alexa FluorTM Plus 488 Thermo Fisher Scientific Cat# A32723;
RRID: AB_2633275
Chicken anti-Mouse IgG (H + L) Cross-adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat# A-21200;
RRID: AB_2535786
Donkey anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 Thermo Fisher Scientific Cat# A-31573;
RRID: AB_2536183
Donkey anti-Goat IgG (H + l) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 568 Thermo Fisher Scientific Cat#: A-11057;
RRID: AB_2534104
Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat# A-11008;
RRID: AB_143165
Donkey anti-Goat IgG (H + L) Antibody, Alexa Fluor® 633 Thermo Fisher Scientific Cat#A21082;
RRID: AB_10562400
Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 633 Thermo Fisher Scientific Cat# A-21050;
RRID: AB_2535718
Anti-Pitx2ABC Capra Science Cat# PA-1020;
RRID: AB_2819162
Goat anti-Mouse IgG (H + L) Secondary Antibody, HRP Thermo Fisher Scientific Cat# 32430;
RRID: AB_1185566
Mouse anti-rabbit IgG-HRP Santa Cruz Biotechnology Cat# sc-2357;
RRID: AB_628497
Recombinant Anti-Ferritin Heavy Chain antibody Abcam Cat# AB183781;
RRID: AB_2940987
Bacterial and virus strains
NEB® Stable Competent E. coli New England Biolabs Inc. C3040H
NEB® 5-alpha Competent E. coli New England Biolabs Inc. C2987H
Chemicals, peptides, and recombinant proteins
Tamoxifen SigmaAldrich Cat# T5648
CAS: 10540-29-1
IWR-1-endo SelleckChem Cat# S7086
CAS: 1127442-82-3
Ammonium iron (II) sulfate hexahydrate 98.5–101.5% ACS Thermo Fisher Scientific Cat# AA13448-36
CAS:7783-85-9
Erastin MilliporeSigma Cat# E7781
CAS: 571203-78-6
Picric Acid Solution SigmaAldrich Cat# P6744
CAS: 88-89-1
Acetic acid Sigma-Aldrich Cat# A6283
CAS: 64-19-7
Direct Red Sigma-Aldrich Cat# 365548
CAS: 2610-10-8
DAPI Sigma-Aldrich Cat# D9542
CAS: 28718-90-3
Bovine Serum Albumin Sigma-Aldrich Cat# A3059
CAS: 9048-46-8
TritonTM X-100 Sigma-Aldrich Cat# T8787
CAS: 9036-19-5
Ponceau S Sigma-Aldrich Cat# P3504
CAS: 6226-79-5
Ferrous Ammonium Sulfate Thermo Fisher Scientific Cat# A15178.36
CAS: 7782-63-0
Critical commercial assays
Proteome Profiler Human XL Cytokine Array Kit R and D Systems ARY022B
MilliporeSigma PROTEIN-CONCENTRATE Kit Thermo Fisher Scientific 50-525-36
PierceTM BCA Protein Assay Kit Thermo Fisher Scientific 23225
Gibson Assembly Kit New England BioLabs E2611
AAVpro® Purification Kit Midi (All Serotypes) Takara Bio USA, Inc. 6675
Deposited data
Raw and Analyzed Data This paper GEO: GSE211568
Experimental models: Cell lines
HEK293 ATCC Cat# CRL-1573,
RRID: CVCL_0045
AC16 (Human hybrid cardiomyocyte) MilliporeSigma RRID: CVCL_4U18
WTC-mEGFP-TTN-cl39 Coriell Institute for Medical Research RRID: CVCL_UD16
Primary Human Cardiac Fibroblasts (HCF) Cell Applications Cat# 306V-05A
N/A
AAVpro® 293T cell Line Takara Bio USA, Inc. Cat# 632273
Lenti-X 293T Clontech Laboratories, Inc. Cat# 632180
Experimental models: Organisms/strains
Mouse:FVB/NJ The Jackson Laboratory RRID: IMSR_JAX:001800
Mouse: C57BL/6J The Jackson Laboratory RRID: IMSR_JAX:000664
Mouse: B6.FVB(129)-A1cfTg(Myh6−cre/Esr1∗)JmK/J The Jackson Laboratory RRID: IMSR_JAX:005657
Mouse: B6.129S-Pdgfratm1.1(cre/ERT2)Blh/J The Jackson Laboratory RRID: IMSR_JAX:032770
Mouse: B6.129P2-Gt(ROSA)26Sortm1(DTA)Lky/J The Jackson Laboratory RRID: IMSR_JAX:009669
Mouse: B6.129S-Postntm2.1(cre/Esr1∗)Jmol/J The Jackson Laboratory RRID: IMSR_JAX:029645
Mouse: B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,−EGFP)Luo/J The Jackson Laboratory RRID: IMSR_JAX:007676
Mouse: Mhccre-Er The Jackson Laboratory IMSR_JAX:005657
Mouse: Pitx2GoF Tao et al., Nature 201636 N/A
Mouse: Pitx2f/f Gage PJ et al., Development 199959 N/A
Mouse: Myh6Cas9 Carroll et al., PNAS 201626 N/A
Oligonucleotides
siRNA targeting sequence FTH1 (hs.Ri.FTH1.13.2)
rGrUrUrUrArCrCrUrGrUrCrCrArUrGrUrCrUrUrArCrUrAC U rArGrUrArGrUrArArGrArCrArUrGrGrArCrArGrGrUrArArArCrGrU		 Integrated DNA Technologies Cat# hs.Ri.FTH1.13.2
siRNA targeting sequence FTL (hs.Ri.FTL.13.1)
rGrArGrGrArArGrUrGrArArGrCrUrUrArUrCrArArGrArAGA rUrCrUrUrCrUrUrGrArUrArArGrCrUrUrCrArCrUrUrCrCrUrCrArU		 Integrated DNA Technologies Cat# hs.Ri.FTL.13.1
siRNA targeting sequence CX43 (hs.Ri.GJC1.13.1)
rArGrArArUrGrGrArCrUrUrArCrArUrGrUrUrArUrCrArGUU rArArCrUrGrArUrArArCrArUrGrUrArArGrUrCrCrArUrUrCrUrUrC		 Integrated DNA Technologies Cat# hs.Ri.GJC.13.1
siRNA targeting sequence CX45 (hs.Ri.GJA1.13.2)
rGrUrGrGrUrArCrArUrCrUrArUrGrGrArUrUrCrArGrCrUUG rCrArArGrCrUrGrArArUrCrCrArUrArGrArUrGrUrArCrCrArCrUrG		 Integrated DNA Technologies Cat# hs.Ri.GJA1.13.2
Scrambled Negative Control
rCrUrUrCrCrUrCrUrCrUrUrUrCrUrCrUrCrCrCrUrUrGrUGA rUrCrArCrArArGrGrGrArGrArGrArArArGrArGrArGrGrArArGrGrA		 Integrated DNA Technologies Cat# 51-01-19-08
Cat# 51-01-19-09
Recombinant DNA
AAV-U6-GPX4gRNA1-U6gGPX4RNA2-TnT-Cre This paper N/A
AAV-U6gRNA1-U6gRNA2-TnT-Cre Addgene 87682
pAAV2/9n Addgene 112865
pAdDeltaF6 Addgene 112867
pWPI Addgene 12254
pMD2.G Addgene 12259
psPAX2 Addgene 12260
Software and algorithms
ImageJ NIH RRID: SCR_00370
https://imagej.net/software/
STAR (Spliced Transcripts Alignment to a Reference) Dobin et al.60 RRID:SCR_004463
DESeq2 Love et al.61 RRID:SCR_015687
Graphpad Prism GraphPad Software RRID:SCR_002798
https://www.graphpad.com/
R R core team https://www.r-project.org/