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Enzymatic ssDNA production through PaNDA. (A) Reaction scheme. The input DNA sequences used in our current study are shown in Table S1 in Supporting Information (SI). (B) Polyacrylamide gel electrophoresis (PAGE) for PaNDA of a 15-nt ssDNA oligonucleotide. DNA was visualized by SYBR Gold (Invitrogen). (C) Resource-Q anion-exchange chromatogram for the PaNDA reaction mixture to produce the 15-nt ssDNA.
