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. Author manuscript; available in PMC: 2025 Jan 24.
Published in final edited form as: J Am Chem Soc. 2024 Jan 8;146(3):1748–1752. doi: 10.1021/jacs.3c11219

Figure 4.

Figure 4.

Application of PaNDA to Dz5C, a 33-nt DNAzyme. (A) Confirmation of RNA cleavage by PaNDA-produced Dz5C in the presence of Mg2+. Lane 1 is for a substrate 19-nt RNA; Lane 2, chemically synthesized Dz5C; Lane 3, Dz5C produced through PaNDA; Lane, 4, the mixture of Dz5C and the RNA substrate in the absence of any divalent ions; Lane 5, the reaction mixture of the RNA substrate and the chemically synthesized Dz5C; and Lane 6, the reaction mixture of the RNA substrate and the Dz5C produced through PaNDA. DNA and RNA were visualized by SYBR Gold. (B) Overlaid 1H-13C HSQC NMR spectra recorded for 13C,15N-labeled Dz5C in the free state and in the complex with the RNA substrate in the absence of divalent metal ions.