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. Author manuscript; available in PMC: 2024 Mar 11.
Published in final edited form as: J Mol Evol. 2021 Dec 29;90(1):30–43. doi: 10.1007/s00239-021-10039-9

Fig. 5. The three TnI isoforms found in lungfish.

Fig. 5.

Coomassie Blue-stained SDS-gel of South American lungfish (Lepidosiren paradoxa) cardiac and skeletal muscles and Western blots using a pan-TnI mAb TnI-1 which recognizes a highly conserved C-terminal epitope (Jin et al., 2001) showed that lungfish possess three TnI isoforms. A parallel blot with mAb CT3 against cardiac and slow skeletal muscle TnT, followed by reblotting of the same membrane with a pan-TnT mAb 2C8, demonstrates the co-presence of cardiac, fast and slow skeletal muscle TnT isoforms and multiple alternative splice forms in lungfish muscles.