Skip to main content
. 2022 Jul 22;2:959077. doi: 10.3389/finsc.2022.959077

Figure 2.

Figure 2

Dot blot analysis of dsRNA accumulation in Hi5 cells that were infected with the recombinant baculoviruses AcMNPV-GFP (1), AcMNPV-dsLuc/GFP (2), AcMNPV-dsLuc/B2-GFP (3) and AcMNPV-asLuc/B2-GFP (4) at 3 days post infection (dpi). (A) displays a representative dot blot of both soluble and insoluble cellular extracts. (B, C) present the quantification of dot blot signals following repeat experimentation (N =3) for soluble and insoluble extracts, respectively. P values indicate the significance for the comparison with uninfected Hi5 cells (paired t-test). D compares the signal of 1 and 10 ng of in vitro transcribed dsLuc or dsRNA genome of Bombyx mori Cypovirus (CPV) with the signal of 1/20 of the soluble extract of Hi5 cells infected with AcMNPV-dsLuc/B2-GFP (corresponding to 25,000 cells; 2 repeats), as indicated. (E) shows recombinant protein expression [GFP (30 kDa) and B2-GFP (39 kDa)] in the cellular media by western blot and the accumulation of baculovirus genomes and dsLuc in the insoluble cellular extracts [PCR detection of gp64 (320 bp) and dsLuc stem sequence (398 bp)]. The exposure time for the dot blot in (A–D) is 5 minutes and 1 minute, respectively. dsL, dsLuc; asL, antisense Luc.