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. 2022 Jul 22;2:959077. doi: 10.3389/finsc.2022.959077

Figure 8.

Figure 8

Fluorescence in situ hybridization for detection of the loop of dsLuc in Hi5 cells infected with the recombinant baculoviruses AcMNPV-dsLuc/B2-GFP and AcMNPV-dsLuc-MBS/tdMCP-GFP viruses, as indicated, at 4 days post infection (dpi). Double staining of the proteins B2-GFP and tdMCP-GFP was also performed by immunostaining using anti-Myc antibody. Shown are white light (WL), Alexa Fluor® 594 (sense or antisense loop), Alexa Fluor® 488 (B2-GFP or tdMCP-GFP), DAPI and Alexa Fluor® 594/Alexa Fluor® 488 overlay images. Overlap of signals between FISH and immunostaining are indicated by arrows. FISH signals obtained with the antisense probe showed much higher intensity compared with the sense probe in the infected cells. There was only limited overlap between RNA hairpin loop detection (FISH) and the localization of dsRNA (detection of B2-GFP and tdMCP-GFP). The white bar in the photographs corresponds to a length of 10 μm.