Figure 2.

Knockdown of dsRNase1 improved dsRNA stability in conditioned medium from Sf9_SID1_Luciferase cells. Sf9_SID1_Luciferase cells seeded in 48 well plates were treated with 1 µg dsRNA targeting different nuclease genes. After two days of incubation, fresh medium was replaced, and incubated for two additional days. The medium was collected and used in dsRNA degradation assay. One µg dsGFP was incubated with 19 µl of the conditioned medium at 28°C for 5 hours and the products were run on 1% agarose gels. dsGFP incubated with water (Water), Sf-900 medium (Medium), conditioned medium from untreated cells (Untreated), conditioned medium from dsRNA treated cells targeting GFP (dsGFP), dsRNase1 (dsdsRNase1), REase (dsREase), and RNaseT2 (dsRNaseT2).