FIG. 8.

Localization of the Cdic-GFP fusion proteins in cultured Schneider-3 cells. A schematic representation of the full-size fusion and N-terminal fusion proteins is shown at the top. Cells were transfected with plasmids expressing fusion proteins under the control of the cytomegalovirus promoter and stained with propidium iodide. Staining of the cellular content with propidium iodide was detected in the rhodamine channel, and the image was converted to the contour of the cell. GFP fluorescence was detected in the fluorescein isothiocyanate channel and pseudocolored in white. IC isoforms are indicated; for example, Cdic1a:GFP is a full-size fusion of Cdic1a isoform with GFP, and N-Cdic2b and N-Cdic2c are the N-terminal fusions. The localization of the GFP expressed alone is shown for comparison.