TABLE 2.
Representation of isoforms in Cdic mRNAs from different sourcesa
| Cdic isoform | % of Cdic mRNA inb:
|
|||||
|---|---|---|---|---|---|---|
| Embryo | Fly | Ovary | Midgut | Head | Torso | |
| Constitutive | ||||||
| 1a | 41 ± 3 | 54 ± 4 | 43 ± 1 | 51 ± 1 | 36 ± 6 | 49 ± 5 |
| 2a | 27 ± 4 | 28 ± 5 | 22 ± 1 | 24 ± 5 | 21 ± 1 | 27 ± 4 |
| 2b | 15 ± 2 | 20 ± 4 | 11 ± 2 | 16 ± 3 | 11 ± 1 | 17 ± 2 |
| 2c | 4 ± 1 | 4 ± 1 | 6 ± 3 | 4 ± 1 | 3 ± 2 | 2 ± 2 |
| Ovary-specific | ||||||
| 1b+4 | 10 ± 4 | 8 ± 2 | 17 ± 2 | 2 ± 1 | ND | ND |
| 1c | 3 ± 1 | 1 ± 1 | 3 ± 2 | ND | ND | ND |
| 3a | 4 ± 1 | 2 ± 1 | 8 ± 2 | ND | ND | ND |
| Head-specific | ||||||
| 5a | NDc | 1 ± 1 | ND | ND | 11 ± 4 | 2 ± 1 |
| 5b | 3 ± 2 | 3 ± 1 | ND | ND | 23 ± 3 | 6 ± 1 |
a
Cdic RT-PCR products amplified from the RNA extracted from indicated sources were end-labeled with 32P and separated in a sequencing gel, as shown in Fig. 7. The gel was exposed to Biomax-MR film (Kodak), the film was scanned, and the image was analyzed with Biomax-1D software (Kodak) to quantitate the intensity of the bands.
b
Representation of the isoform in the total mRNA pool was calculated by normalizing the intensity of relevant band by the total intensity of all specific bands on the lane. The numbers indicate the mean and standard error of results obtained in three experiments.
c
ND, nondetectable.