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. 2023 May 12;3:1151789. doi: 10.3389/finsc.2023.1151789

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Copyright © 2023 Carroll, Kunte, McGraw, Gautam, Range, Noveron-Nunez, Held and Avila

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Stability assessment of BAPCs and BAPC-dsRNA complexes in a buffer mimicking P. japonica gut (pH 7.4). (B) BAPC loading capacity assessed by the electrophoretic mobility shift assay. BAPC-dsRNA complexes were formed by mixing 20μM and 60μM BAPCs with 1μg dsRNA. Controls containing only 20μM or 60μM BAPCs without dsRNA were also run to show they did not produce background signal. Differences between values were compared by one way ANOVA using Tukey as post-test. Statistical significance: (*) P < 0.05; (***) P < 0.001. Non-statistical significance (ns) was considered when P > 0.05.