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. 2021 Jul 30;1:696179. doi: 10.3389/finsc.2021.696179

Figure 2.

Figure 2

Expression levels of identified PxutOBPs. (A) Appendages examined for gene expression with a magnified view of the foreleg tarsus. Orange brushes in the dotted ellipse are the gustatory sensilla. As few gustatory sensilla are distributed in the first tarsomere (11), we collected the other four tarsomeres as “leg tarsus” samples. (B) Comparison of expression levels of OBPs between the tarsus of the forelegs and that of the other legs sampled from 0-day-old adult females performed by qRT-PCR. Three individuals were used per lot. All data are shown as the means ± SEM (n = 5, each leg). Student's t-test was conducted. OBP genes expressed significantly higher in foreleg tarsus (p < 0.05) or exhibiting prominent average fold change (the ratio of expression levels in foreleg tarsus to those in the other leg tarsus > 5) were indicated by orange-colored numbers. (C) Comparison of expression levels of OBPs among the foreleg tarsus (FLt), midleg tarsus (MLt), hindleg tarsus (HLt), antenna (An), proboscis (Pb), and ovipositor (Ovp) sampled from 0-day-old adult females performed by qRT-PCR. Two individuals were used per lot. All data are shown as the means ± SD (n = 6, each appendage). Multiple comparisons were performed using Dunnett's test. Asterisks indicate significant differences compared to expression levels of the foreleg tarsus (*, p < 0.05; **, p < 0.01).