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Primers used for constructing the mutant and the complemented strain
| Primer | Sequence (5’ − 3’) |
|---|---|
| P1: | TGGGCATGGTCAATATTCGGGATCAGGTCATTCCGGTGATTGATTTGCCAGCGGTAGCGTGTGTAGGCTGGAGCTGCTTCG |
| P2: | TCATTGGCGCTGCCGGAAAGCGAGGAGTGGATAACGACCGGGATCTTTTTCAGCCGTTCCATATGAATATCCTCCTTAG |
| P3: | TGAACTGCTGTTATTCCGTCTTGG |
| P4: | ATCAGTGGTCCCTGGCTGTTTG |
| P5: | GTTAGGATGGACAATTTTCAGAAAGATA |
| P6: | TCAGGCGCTTTTGCGGCTGATCAGTGGT |
